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Research Project: IPM Methods for Insect Pests of Orchard Crops

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Title: Improved annotation of the insect vector of citrus greening disease: biocuration by a diverse genomics community

Author
item SAHA, SURYA - Boyce Thompson Institute
item HOSMANI, PRASHANT - Boyce Thompson Institute
item BILLALOBOS-AYALA, KRYSTAL - Indian River State College
item MILLER, SHERRY - Kansas State University
item SHIPPY, TERESA - Kansas State University
item FLORES, MIRELLA - Boyce Thompson Institute
item ROSENDALE, ANDREW - University Of Cincinnati
item CORDOLA, CHRIS - Indian River State College
item BELL, TRACEY - Indian River State College
item MANN, HANNAH - Indian River State College
item DEAVILA, GABE - Indian River State College
item DEAVILA, DANIEL - Indian River State College
item MOORE, ZACHARY - University Of Cincinnati
item BULLER, KYLE - University Of Cincinnati
item CIOLKEVICH, KATHRYN - University Of Cincinnati
item NANDYAL, SAMANTHA - University Of Cincinnati
item MAHONEY, ROBERT - University Of Cincinnati
item VAN VOORHIS, JOSHUA - University Of Cincinnati
item DUNLEVY, MEGAN - University Of Cincinnati
item FARROW, DAVID - University Of Cincinnati
item HUNTER, DAVID - Kansas State University
item MORGAN, TAYLAR - Kansas State University
item SHORE, KAYLA - Kansas State University
item GUZMAN, VICTORIA - Kansas State University
item IZSAK, ALLISON - Cornell University
item DIXON, DANIELLE - Boyce Thompson Institute
item CRIDGE, ANDREW - New Zealand Institute For Crop & Food Research
item CANO, LILIANA - University Of Florida
item CAO, XIAOLONG - Oklahoma State University
item JIANG, HAOBO - Oklahoma State University
item LENG, NAN - Illumina, Inc
item JOHNSON, SHANNON - University Of New Mexico
item CANTAREL, BRANDI - Utah Health Science Center
item RICHARDS, STEPHEN - Baylor College Of Medicine
item ENGLISH, ADAM - Baylor College Of Medicine
item Shatters, Robert - Bob
item Childers, Christopher
item CHEN, MEI-JU - National Central University - Taiwan
item Heck, Michelle
item MUELLER, LUKAS - Cornell University
item MUNOZ-TORRES, MONICA - Lawrence Berkeley National Laboratory
item NELSON, DAVID - University Of Tennessee
item Poelchau, Monica
item BENOIT, JOSHUA - University Of Cincinnati
item WIERSMA-KOCH - Indian River State College
item BROWN, SUSAN - Kansas State University

Submitted to: bioRxiv
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/23/2016
Publication Date: 1/9/2017
Citation: Saha, S., Hosmani, P.S., Billalobos-Ayala, K., Miller, S., Shippy, T., Flores, M., Rosendale, A., Cordola, C., Bell, T., Mann, H., DeAvila, G., DeAvila, D., Moore, Z., Buller, K., Ciolkevich, K., Nandyal, S., Mahoney, R., Van Voorhis, J., Dunlevy, M., Farrow, D., Hunter, D., Morgan, T., Shore, K., Guzman, V., Izsak, A., Dixon, D.E., Cridge, A., Cano, L., Cao, X., Jiang, H., Leng, N., Johnson, S., Cantarel, B.L., Richards, S., English, A., Shatters, R.G., Childers, C., Chen, M., Cilia, M., Mueller, L., Munoz-Torres, M., Nelson, D., Poelchau, M.F., Benoit, J., Wiersma-Koch, Brown, S.J. 2017. Improved annotation of the insect vector of citrus greening disease: biocuration by a diverse genomics community. bioRxiv. https://doi.org/10.1093/database/bax032.
DOI: https://doi.org/10.1093/database/bax032

Interpretive Summary: The Asian citrus psyllid (Diaphorina citri Kuwayama) is the insect vector of the bacterium Candidatus Liberibacter asiaticus (CLas), the pathogen associated with citrus Huanglongbing (HLB, citrus greening). HLB threatens citrus production worldwide. Suppression or reduction of the insect vector using chemical insecticides has been the primary method to inhibit the spread of citrus greening disease. Accurate structural and functional annotation of the Asian citrus psyllid genome, as well as a clear understanding of the interactions between the insect and CLas, are required for development of new molecular-based HLB control methods.

Technical Abstract: The Asian citrus psyllid (Diaphorina citri Kuwayama) is the insect vector of the bacterium Candidatus Liberibacter asiaticus (CLas), the pathogen associated with citrus Huanglongbing (HLB, citrus greening). HLB threatens citrus production worldwide. Suppression or reduction of the insect vector using chemical insecticides has been the primary method to inhibit the spread of citrus greening disease. Accurate structural and functional annotation of the Asian citrus psyllid genome, as well as a clear understanding of the interactions between the insect and CLas, are required for development of new molecular-based HLB control methods. This community effort produced 530 manually curated gene models across developmental, physiological, Ribonucleic acid interference (RNAi) regulatory, and immunity-related pathways. As previously shown in the pea aphid, RNAi machinery genes putatively involved in the microRNA (Ribonucleic acid) pathway have been specifically duplicated. A comprehensive transcriptome enabled us to identify a number of gene families that are either missing or misassembled in the draft genome. In order to develop biocuration as a training experience, we included undergraduate and graduate students from multiple institutions, as well as experienced annotators from the insect genomics research community. The resulting gene set (OGS v1.0)combines both automatically predicted and manually curated gene models. All data are available on https://citrusgreening.org/.