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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Plant Stress and Germplasm Development Research » Research » Publications at this Location » Publication #344964
Title: Transcriptome response to elevated CO2, water deficit, and thermal stress in peanut

Author
item LAZA, HAYDEE - Texas Tech University
item Mahan, James
item Baker, Jeffrey
item Gitz, Dennis
item TILLMAN, BARRY - University Of Florida
item ROWLAND, DIANE - University Of Florida
item TISSUE, DAVID - Western Sydney University
item Holbrook, Carl - Corley
item KOTTAPALLI, RAO - Texas Tech University
item Payton, Paxton

Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 8/1/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Previously, our laboratories have performed gene expression studies using EST sequencing and spotted microarrays to investigate tissue-specific gene expression and response to abiotic stress. While these studies have provided valuable insight into these processes, they are constrained by sequencer throughput, expense, or require a priori information about gene sequence and function. RNA sequencing (RNA-seq) is an open architecture gene expression technology that eliminates many of these problems and allows for the identification of new gene sequences while providing robust gene expression information. We examined the interactive effects of long term exposure to elevated [CO2] (ambient + 250ppm) and water deficit on physiological and molecular responses, in a peanut (C-7616) runner market type, grown in field conditions. A comparative leaf transcriptomic profile across three periodic water deficit/re-hydration cycles through the growing season was evaluated using RNA-seq analysis. Six data points (pre-water stress, early and late water deficit, and re-hydration events were selected. Results show significantly differential gene expression between and within [CO2] treatments at different water availability stages. Mercator annotation analysis shows that lipid metabolism, hormone biosynthesis, secondary metabolism, amino acid biosynthesis and transport, are among the most impacted biological processes by the combined abiotic stresses addressed in this study. Overall, the number of differentially expressed genes increased overtime, and was more pronounced in plants grown at ambient versus elevated [CO2] conditions. A detailed analysis of differential expression by treatment and stages will be presented.