Medium optimization for production of anti-streptococcal liamocins by Aureobasidium pullulans

Authors: Leathers, Timothy; Skory, Christopher - Chris; Price, Neil; Nunnally, Melinda

Submitted to: Biocatalysis and Agricultural Biotechnology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/17/2017
Publication Date: 11/22/2017
Publication URL: https://handle.nal.usda.gov/10113/5871063
Citation: Leathers, T.D., Skory, C.D., Price, N.P.J., Nunnally, M.S. 2017. Medium optimization for production of anti-streptococcal liamocins by Aureobasidium pullulans. Biocatalysis and Agricultural Biotechnology. 13:53-57. doi: 10.1016/j.bcab.2017.11.008.
DOI: https://doi.org/10.1016/j.bcab.2017.11.008

Interpretive Summary: Previous studies identified growth medium for production of "liamocins," promising new antimicrobial compounds produced by the fungus Aureobasidium pullulans. In this research, statistical methods were used to optimize growth medium for liamocin production. Liamocin yields from optimized medium were nearly double those from standard medium. This work will facilitate the development of liamocins as new antimicrobial agents.

Technical Abstract: Liamocins are antimicrobial compounds with specificity for Streptococcus spp., produced by certain strains of the fungus Aureobasidium pullulans. Recent studies have identified strains and culture medium for liamocin production. However, this culture medium has not previously been optimized for liamocin production. In this study, we employ one-factor-at-a-time and statistical methods to develop an optimized medium for liamocin production. Liamocin yields from optimized medium were nearly double those from standard medium, up to 22 g liamocin/L. This represents the highest yields of liamocin reported to date. Furthermore, MALDI-TOF analyses show that liamocins produced from optimized medium are similar to those produced from standard medium.