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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Hard Winter Wheat Genetics Research » Research » Publications at this Location » Publication #345362
Title: QTL mapping of pre-harvest sprouting resistance in white wheat cultivar Danby

Author
item SHAO, MINGQIN - Kansas State University
item Bai, Guihua
item RIFE, TREVORE - Kansas State University
item POLAND, JESSE - Kansas State University
item LIN, MENG - Kansas State University
item LIU, SHUBING - Kansas State University
item KUMSSA, TADELE - Kansas State University
item ZHANG, GUORONG - Kansas State University

Submitted to: Journal of Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/2/2018
Publication Date: 6/2/2018
Citation: Shao, M., Bai, G., Rife, T., Poland, J., Lin, M., Liu, S., Kumssa, T., Zhang, G. 2018. QTL mapping of pre-harvest sprouting resistance in white wheat cultivar Danby. Journal of Theoretical and Applied Genetics. 131:1683-1697.

Interpretive Summary: Pre-harvest sprouting (PHS) causes significant losses in grain yield and end-use quality. Usually white wheat is preferred for Asian noodles and steamed breads, but it is more susceptible to PHS than red wheat. Danby is a white wheat cultivar and has a high level of PHS resistance. We found that one major gene on the short arm of chromosome 3A of Danby explained 20.12 to 43.63% of the sprouting variation. This gene is the same as a previously cloned gene, TaPHS1. In addition, two new minor genes for PHS resistance were detected on the chromosome arms 3BS and 5AL. DNA markers for all three genes were developed for marker-assisted selection for PHS resistance in future cultivars of white wheat.

Technical Abstract: Although white wheat is preferred for Asian noodles and steamed breads, it is usually more susceptible to PHS than red wheat. Therefore, use of non-grain color-related PHS resistance quantitative trait loci (QTLs) is essential for improvement of PHS resistance in white wheat. To identify PHS QTLs in a white wheat cultivar, Danby, and determine their effects, a doubled haploid (DH) population derived from a cross of Danby × Tiger was genotyped using genotyping-by-sequencing (GBS) markers and phenotyped for PHS resistance in both greenhouse and field experiments. One major QTL was consistently detected on the short arm of chromosome 3A in all three experiments and explained 20.12 to 43.63% of the phenotypic variation. This QTL corresponds to a previously cloned gene, TaPHS1. A SNP in the promoter of TaPHS1 co-segregated with PHS resistance in this population. In addition, two minor QTLs were detected on chromosome arms 3BS and 5AL in two experiments. The two minor QTLs together with TaPHS1 showed significant synergistic effects. The results demonstrated that pyramiding those three QTLs from Danby could greatly improve PHS resistance in white wheat using SNP markers developed in this study.