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Title: The P450 gene CYP749A16 is required for tolerance to the sulfonylurea herbicide trifloxysulfuron sodium in cotton (Gossypium hirsutum L.)

Author
item Thyssen, Gregory
item Naoumkina, Marina
item McCarty, Jack
item Jenkins, Johnie
item Florane, Christopher
item Li, Ping
item Fang, David

Submitted to: BMC Plant Biology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/31/2018
Publication Date: 9/11/2018
Citation: Thyssen, G.N., Naoumkina, M.A., McCarty, J.C., Jenkins, J.N., Florane, C.B., Li, P., Fang, D.D. 2018. The P450 gene CYP749A16 is required for tolerance to the sulfonylurea herbicide trifloxysulfuron sodium in cotton (Gossypium hirsutum L.). Biomed Central (BMC) Plant Biology. 18:186. https://doi.org/10.1186/s12870-018-1414-2.
DOI: https://doi.org/10.1186/s12870-018-1414-2

Interpretive Summary: Weed management is critical to global crop production and is complicated by rapidly evolving herbicide resistance in weeds. New sources of herbicide resistance are needed for crop plants so that applied herbicides can be rotated or combined to thwart the evolution of resistant weeds. Here, we report the identification of CYP749A16 (Gh_D10G1401) which is responsible for the natural tolerance exhibited by most cotton cultivars to the herbicide trifloxysulfuron sodium (CGA 362622, commercial name Envoke). The 1-bp frameshift insertion in the third exon of CYP749A16 results in the loss of resistance to Envoke herbicide. The DNA marker designed from this insertion perfectly co-segregated with the phenotype in 2145 F2 progeny of a cross between the susceptible cultivar Paymaster HS26 and tolerant cultivar Stoneville 474, and in 550 recombinant inbred lines of a multi-parent advanced generation inter-cross population. Marker analysis of 382 additional cotton cultivars identified twelve cultivars containing the 1-bp frameshift insertion. A greenhouse experiment demonstrated a perfect match between marker genotypes and phenotypes in 188 plants from the selected twelve cultivars. Virus induced gene silencing of CYP749A16 generated sensitivity in the resistant cotton cultivar Stoneville 474. Transformation of Arabidopsis with GhCYP749A16 did not generate herbicide resistance, which may be related to the loss of the entire CYP749 family or unknown cofactors in Arabidopsis. Taken together, we conclude that CYP749A16 is required for resistance in cotton, but is not sufficient for Envoke herbicide resistance in Arabidopsis.

Technical Abstract: Weed management is critical to global crop production and is complicated by rapidly evolving herbicide resistance in weeds. New sources of herbicide resistance are needed for crop plants so that applied herbicides can be rotated or combined to thwart the evolution of resistant weeds. The diverse family of cytochrome P450 proteins has been suggested to be a source of detoxifying herbicide metabolism in both weed and crop plants, and greater understanding of these genes will offer avenues for crop improvement and novel weed management practices. Here, we report the identification of CYP749A16 (Gh_D10G1401) which is responsible for the natural tolerance exhibited by most cotton, Gossypium hirsutum L., cultivars to the herbicide trifloxysulfuron sodium (CGA 362622, commercial name Envoke). The 1-bp frameshift insertion in the third exon of CYP749A16 results in the loss of resistance to Envoke herbicide. The DNA marker designed from this insertion perfectly co-segregated with the phenotype in 2145 F2 progeny of a cross between the susceptible cultivar Paymaster HS26 and tolerant cultivar Stoneville 474, and in 550 recombinant inbred lines of a multi-parent advanced generation inter-cross population. Marker analysis of 382 additional cotton cultivars identified twelve cultivars containing the 1-bp frameshift insertion. A greenhouse experiment demonstrated a perfect match between marker genotypes and phenotypes in 188 plants from the selected twelve cultivars. Virus induced gene silencing of CYP749A16 generated sensitivity in the resistant cotton cultivar Stoneville 474. Transformation of Arabidopsis with GhCYP749A16 did not generate herbicide resistance, which may be related to the loss of the entire CYP749 family or unknown cofactors in Arabidopsis. Taken together, we conclude that CYP749A16 is required for resistance in cotton, but is not sufficient for Envoke herbicide resistance in Arabidopsis.