Establishing and performing targeted multi-residue analysis for lipid mediators and fatty acids in small clinical plasma samples.

Authors: PEDERSEN, THERESA - Advanced Analytical Tech, Inc; Newman, John

Submitted to: Methods in Molecular Biology
Publication Type: Book / Chapter
Publication Acceptance Date: 9/28/2017
Publication Date: 2/21/2018
Citation: Pedersen, T.L., Newman, J.W. 2018. Establishing and performing targeted multi-residue analysis for lipid mediators and fatty acids in small clinical plasma samples. In: Giera, M, editor. Clinical Metabolomics. Methods in Molecular Biology. Volume 1730. New York, NY: Humana Press. p. 175-212. https://doi.org/10.1007/978-1-4939-7592-1_13
DOI: https://doi.org/10.1007/978-1-4939-7592-1_13

Interpretive Summary: The term metabolomics refers to modern analytical chemistry approaches measuring vast arrays of metabolites in biological samples at the same time. By developing methods which target specific metabolic pathways, metabolomics approaches can be made quantitative, and thus amenable to the use in clinical environments. This book chapter provides step-by-step protocols for the development of multi-residue analytical profiles, calibration standards, and internal standard solutions in support of a fast, simple, and low cost plasma sample preparation method to capture and quantify multiple metabolite cascades using gas and liquid chromatography with mass spectrometry based detection. The simultaneous quantification of an array of ~150 bioactive lipids, bile acids, and fatty acids are described.

Technical Abstract: LC-MS/MS and GC-MS based targeted metabolomics is typically conducted by analyzing and quantifying a cascade of metabolites with methods specifically developed for the metabolite class. Here we describe an approach for the development of multi-residue analytical profiles, calibration standards, and internal standard solutions in support of a fast, simple, and low cost plasma sample preparation that captures and quantitates a range of metabolite cascades.