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ARS Home » Pacific West Area » Davis, California » Crops Pathology and Genetics Research » Research » Publications at this Location » Publication #346549
Research Project: Integrated Disease Management Strategies for Woody Perennial Species

Location: Crops Pathology and Genetics Research

Title: Draft genome sequence of agrobacterium tumefaciens biovar 1 strain 186, isolated from walnut

Author
item Poret-Peterson, Amisha
item BHATNAGAR, SRIJAK - University Of California
item McClean, Ali
item Kluepfel, Daniel

Submitted to: Genome Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/23/2017
Publication Date: 11/16/2017
Citation: Poret-Peterson, A.T., Bhatnagar, S., McClean, A.E., Kluepfel, D.A. 2017. Draft genome sequence of agrobacterium tumefaciens biovar 1 strain 186, isolated from walnut. Genome Announcements. 5(46):e01232-17. https://doi.org/10.1128/genomeA.01232-17.
DOI: https://doi.org/10.1128/genomeA.01232-17

Interpretive Summary: Problem: Crown gall disease is one of the top yield limiting diseases of walnuts in California. The causative agent, Agrobacterium tumefaciens is ubiquitous and readily infects and causes tumors on the dominant walnut rootstock used in CA English walnut production. Preliminary data has suggested that A. tumefaciens strains infecting walnut are unique. Consequently, many of the primers and other genetic tools developed for standard Agro strains such as C58 are of limited use for our walnut isolates. Accomplishments and contribution to Solving the Problem: The Draft genome sequence reported here will facilitate the development of molecular tools which will be useful in our efforts to enhance crown gall managements strategies for Walnut. In addition, this genomic sequence data will facilitate our efforts to characterize the genetic diversity of this important plant pathogen.

Technical Abstract: Problem: Agrobacterium tumefaciens isolates infecting Juglans regia and Juglans microcarpa are unique which complicates using the standard A. tumefaciens isolates in the development of molecular tools which are useful in our work on crown gall disease of walnuts. Accomplishments and contribution to Solving the Problem: We have sequenced, assembled and annotated the genome of one of our key walnut infecting Agrobacterium tumefaciens isolates.