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ARS Home » Plains Area » Kerrville, Texas » Knipling-Bushland U.S. Livestock Insects Research Laboratory » LAPRU » Research » Publications at this Location » Publication #346797
Title: Acetylcholinesterase of Phlebotomus papatasi (Scopoli): expression and biochemical properties of orthologous organophosphate resistance mutations from mosquitoes

Author
item Temeyer, Kevin
item TONG, FAN - University Of Florida
item CHEN, QIAO-HONG - Virginia Tech
item CARLIER, PAUL - Virginia Tech
item Perez De Leon, Adalberto - Beto
item BLOOMQUIST, JEFFREY - University Of Florida

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/19/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Phlebotomine sand flies are small hematophagous vectors of human and zoonotic leishmaniases present throughout tropical and subtropical areas of the world. Phlebotomus papatasi is a principal vector of human cutaneous leishmaniasis that has presented serious problems for military operations and resident populations in the Middle East and other areas where they are endemic. The acetylcholinesterase (AChE) of P. papatasi (PpAChE) shares substantial sequence identity with AChE of Lutzomyia spp., the New World Sand Fly, as well as AChE1 of important mosquito species. We utilized targeted mutagenesis and baculovirus expression to produce and characterize recombinant PpAChEs containing mutations orthologous to those responsible for mosquito insensitivity to organophosphate. Biochemical properties of the rPpAChEs containing single amino acid substitutions included increased Michaelis–Menten constant (Km) and decreased sensitivity to organophosphate inhibition. Novel synthetic carbamates were screened to identify candidate compounds for improved efficacy.