Enhancing in situ hydrogen peroxide generation of greige cotton nonwoven wound dressings via ascorbate stabilized copper micro- and nano-particles

Authors: Prevost, Nicolette; Edwards, Judson - Vince; Condon, Brian; YAGER, DORNE - Virginia Commonwealth University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/13/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Understanding how wound dressings may be designed to address critical unsolved issues in wound repair and treatment influences the development of dressings and new concepts of promoting healing. The vast majority of commercial dressing materials focus on the physical aspects of wounds, e.g., acting as a physical barrier to further injury, providing an optimal level of moisture, and the removal of excess exudate. Cotton in its many forms remains the most widely used material that forms the basis of wound dressings. Greige cotton (unbleached cotton) is an intact plant fiber with the cuticle and primary cell wall near the outer surface of the cotton fiber containing pectin, peroxidases and trace metals, which are associated with hydrogen peroxide (H2O2) generation during cotton fiber development. When greige cotton is hydroentangled into a nonwoven material, the components of the cotton fiber cuticle are retained. We have recently determined that greige cotton can generate low levels of H2O2 (5–50 micromolar) examining both brown and white cotton varieties with brown cotton producing more in part due to natural polyphenols/ antioxidants present. Low level hydrogen peroxide generation in the range of 5 – 50µM has been characterized as inducing accelerated wound healing associated with enhanced cell signaling and proliferation. This study is a continuation to develop formulations to enhance the peroxide production, controlling release of hydrogen peroxide in white cotton varieties comparable to those observed in untreated brown cotton, and at a level commensurate with enhanced fibroblast proliferation. Here we assess hydrogen peroxide levels produced in a variety of treated nonwoven analogs to retain sustained hydrogen peroxide production in a therapeutic range associated with enhanced cell proliferation.