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ARS Home » Southeast Area » Stoneville, Mississippi » Genomics and Bioinformatics Research » Research » Publications at this Location » Publication #347919
Title: Tomato leaf curl Oman virus and associated Betasatellite causing leaf curl Disease in tomato in Pakistan

Author
item AHMED, NASIM - National Institute Of Biotechnology And Genetic Engineering (NIBGE)
item ZAIDI, SYED - National Institute Of Biotechnology And Genetic Engineering (NIBGE)
item AMIN, IMRAN - National Institute Of Biotechnology And Genetic Engineering (NIBGE)
item Scheffler, Brian
item MANSOOR, SHAHID - National Institute Of Biotechnology And Genetic Engineering (NIBGE)

Submitted to: European Journal of Plant Pathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/16/2021
Publication Date: 2/27/2021
Citation: Ahmed, N., Zaidi, S., Amin, I., Scheffler, B.E., Mansoor, S. 2021. Tomato yellow leaf curl virus and Associated Betasatellite Causing Leaf Curl Disease on Tomato in Pakistan. European Journal of Plant Pathology. https://doi.org/10.1007/s10658-021-02242-7.
DOI: https://doi.org/10.1007/s10658-021-02242-7

Interpretive Summary: Geminiviruses are a serious threat to crops around the world. There are many types of geminiviruses and it is difficult to keep track of them as they have the ability to change their DNA through different mechanisms and often reproduce in various crops with the host range often changing due to changes in their DNA. In addition to the main viral DNA component, some Geminiviruses carry satellite DNAs that alter the pathogenicity of a given virus. In this study, the geminiviruses Tomato yellow leaf curl virus (TYLCV) was uncovered in a tomato plant in Pakistan, specifically the strain TYLCV-PK. However Papaya leaf curl betasatellite (PaLCuB) was also found in the same plant. This is the first reported instance of PaLCuB and TYLCV-PK being found in tomato plant within Pakistan. Experiments in tobacco showed that a plant infected with both PaLCuB and TYLCV-PK had enhanced symptoms and viral accumulation compared to a plant with only TYLCV-PK

Technical Abstract: The ongoing global spread of Tomato yellow leaf curl virus (TYLCV) poses a serious threat to tomato production in all tropical and sub-tropical regions of the world. In March 2017, during a field survey of Sindh Province in Pakistan, tomato (Solanum lycopersicum) plants showing characteristic tomato yellow leaf curl disease (TYLCD)-like symptoms, such as leaf curling, yellowing and stunted plant growth, were observed. For identification and characterization of causative agent, leaf samples were collected and analyzed for the presence of begomovirus and associated satellites, using respective primers. PCR amplicons of expected size were cloned and sequenced; the results indicated the presence of TYLCV-PK and Papaya leaf curl betasatellite (PaLCuB). Defective molecules of TYLCV were also identified. Phylogenetic analysis and sequence demarcation tool (SDT) showed that these are new isolates of TYLCV-PK and PaLCuB. Biolistic inoculation of TYLCV and associated PaLCuB in Nicotiana benthamiana plants showed that PaLCuB results in enhanced symptoms and virus accumulation. This is the first report of TYLCV and associated PaCLuB infecting tomato crop in Pakistan.