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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Genomics and Improvement Laboratory » Research » Publications at this Location » Publication #348513
Research Project: Improving Feed Efficiency and Environmental Sustainability of Dairy Cattle through Genomics and Novel Technologies

Location: Animal Genomics and Improvement Laboratory

Title: Global transcriptome analysis during adipogenic differentiation and involvement of Transthyretin gene in adipogenesis in cattle

Author
item CAI, HANFANG - Northwest Agriculture And Forestry University
item LI, MINGXUN - Northwest Agricultural & Forestry University
item SUN, XIANMEI - Northwest Agriculture And Forestry University
item PLATH, MARTIN - Northwest Agriculture And Forestry University
item Li, Congjun - Cj
item LAN, XIANGYONG - Northwest Agriculture And Forestry University
item LEI, CHUZHAO - Northwest Agriculture And Forestry University
item HUANG, YONGZHEN - Northwest Agriculture And Forestry University
item BAI, YUEYU - Collaborator
item QI, XINGLEI - Collaborator
item LIN, FENGPENG - Collaborator
item CHEN, HONG - Northwest Agriculture And Forestry University

Submitted to: Frontiers in Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/21/2018
Publication Date: 10/18/2018
Citation: Cai, H., Li, M., Sun, X., Plath, M., Li, C., Lan, X., Lei, C., Huang, Y., Bai, Y., Qi, X., Lin, F., Chen, H. 2018. Global transcriptome analysis during adipogenic differentiation and involvement of Transthyretin gene in adipogenesis in cattle. Frontiers in Genetics. 9:463. https://doi.org/10.3389/fgene.2018.00463.
DOI: https://doi.org/10.3389/fgene.2018.00463

Interpretive Summary: The presence of marbling is an important indication of beef quality. However, it is unclear how is the fat deposition controlled by gene activities. In this report, RNA-sequencing to reveal the molecular mechanisms related to the fat development and deposition. The TTR gene was defined to have a positive role in bovine fatty cell development. These results may begin to reveal the molecular mechanisms related to fatty cell development and to provide useful information for the improvement of beef.

Technical Abstract: The presence of marbling plays an essential role in the gustatory quality of beef, and is a great challenge for Chinese beef cattle breeders. However, knowledge about development and deposition of genome-wide molecular that controls bovine adipose remains unclear. In this study, RNA-seq was applied to reveal the molecular mechanisms underlying the adipocyte differentiation in Qinchuan cattle. A total of 18,283 genes and 1,331 novel genes were detected in pre- and mature adipocytes. 470 genes showed significant abundance changes. A protein interaction network was constructed and indicated that collagen might play an important role during bovine adipogenic differentiation. As the most significantly differently expressed down-regulated DEGs, the role of transthyretin (TTR) gene was defined. Since it could promote the expression of PPARgamma and FABP4, and contribute to the formation of lipid droplets, TTR plays a positive role in bovine adipogenic differentiation. This study is the first comprehensive attempt to explore the transcriptional expression during bovine adipocyte differentiation by RNA-seq. Also, TTR was for the first time identified to contribute to bovine adipogenic differentiation. These results may have begun to reveal the molecular mechanisms underlying adipogenesis and to provide useful information for the improvement of beef.