Genome-wide mapping for stripe rust resistance loci in common wheat cultivar Qinnong 142

Authors: ZEN, QINGDONG - Northwest Agriculture And Forestry University; WU, JIANHUI - Northwest Agriculture And Forestry University; LIU, SHENGJIE - Northwest Agriculture And Forestry University; Chen, Xianming; YUAN, FENGPING - Northwest Agriculture And Forestry University; SU, PINGPING - Northwest Agriculture And Forestry University; WANG, QILIN - Northwest Agriculture And Forestry University; HUANG, SHUO - Northwest Agriculture And Forestry University; MU, JINGMEI - Northwest Agriculture And Forestry University; HAN, DEJUN - Northwest Agriculture And Forestry University; KANG, ZHENSHENG - Northwest Agriculture And Forestry University

Submitted to: Plant Disease
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/28/2018
Publication Date: 1/15/2019
Citation: Zen, Q., Wu, J., Liu, S., Chen, X., Yuan, F., Su, P., Wang, Q., Huang, S., Mu, J., Han, D., Kang, Z. 2019. Genome-wide mapping for stripe rust resistance loci in common wheat cultivar Qinnong 142. Plant Disease. 103(3):439-447. https://doi.org/10.1094/PDIS-05-18-0846-RE.
DOI: https://doi.org/10.1094/PDIS-05-18-0846-RE

Interpretive Summary: To study the genetic basis and identify quantitative trait loci (QTL) conferring adult plant resistance to stripe rust in Chinese wheat variety Qinnong 142, we developed a recombinant inbred line (RIL) mapping population from a cross between Qinnong 142 and susceptible cultivar Avocet S. The parents and 165 F6 RILs were evaluated for stripe rust response in replicated field tests of six site-year environments in China. The infection type (IT) and disease severity (DS) data were recorded and used as phenotypes. The parents and RILs were genotyped with a 35K single nucleotide polymorphism (SNP) array. Four stable QTL were identified in Qinnong 142 and mapped to chromosomes 1BL, 2AL, 2BL, and 6BS using the method of inclusive composite interval mapping. All of the QTL were significant for both the IT and maximum disease severity (MDS) data. These four QTL explained 61.7% to 85.4% of the phenotype variation. The flanking SNP markers of the four QTL were converted to Kompetitive Allele Specific PCR (KASP) markers, which were confirmed in a collection of 188 wheat varieties. All these KASP markers could be easily adapted for high-throughput marker-assisted selection in breeding for wheat cultivars with stripe rust resistance.

Technical Abstract: To study the genetic basis and identify quantitative trait loci (QTL) conferring adult plant resistance to stripe rust in Chinese wheat variety Qinnong 142, we developed a recombinant inbred line (RIL) mapping population from a cross between Qinnong 142 and susceptible cultivar Avocet S. The parents and 165 F6 RILs were evaluated for stripe rust response in replicated field tests of six site-year environments in China. The infection type (IT) and disease severity (DS) data were recorded and used as phenotypes. The parents and RILs were genotyped with a 35K iSelect single nucleotide polymorphisms (SNP) array. Four stable QTL were identified in Qinnong 142 and mapped to chromosomes 1BL (Qyrqin.nwafu-1BL), 2AL (Qyrqin.nwafu-2AL), 2BL (Qyrqin.nwafu-2BL) and 6BS (Qyrqin.nwafu-6BS) using the method of inclusive composite interval mapping (ICIM). All of the QTL were significant for both the IT and maximum disease severity (MDS) data. These four QTL explained 61.7% to 85.4% of the phenotype variation. The flanking SNP markers of the four QTL were converted to Kompetitive Allele Specific PCR (KASP) markers, which were confirmed in a collection of 188 wheat varieties. All these KASP markers could be easily adapted for high-throughput marker-assisted selection in breeding for wheat cultivars with stripe rust resistance.