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ARS Home » Midwest Area » Ames, Iowa » Corn Insects and Crop Genetics Research » Research » Publications at this Location » Publication #349267
Title: Inheritance of 4-hydroxyphenylpyruvate dioxygenase inhibitor herbicide resistance in an Amaranthus tuberculatus population from Iowa, USA

Author
item KOHLHASE, DANIEL - Iowa State University
item Edwards, Jode
item OWEN, MICHEAL - Iowa State University

Submitted to: Plant Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/8/2018
Publication Date: 6/12/2018
Citation: Kohlhase, D.R., Edwards, J.W., Owen, M. 2018. Inheritance of 4-hydroxyphenylpyruvate dioxygenase inhibitor herbicide resistance in an Amaranthus tuberculatus population from Iowa, USA. Plant Science. 274:360-368. https://doi.org/10.1016/j.plantsci.2018.06.004.
DOI: https://doi.org/10.1016/j.plantsci.2018.06.004

Interpretive Summary: Waterhemp (Amaranthus tuberculatus (Moq.) J.D. Sauer) is a weed prevalent in the Midwest United States and can cause yield losses up to 74% in maize (Zea mays L.) and 56% in soybean (Glycine max (L.) Merr.). An important trait found in waterhemp is the ability to evolve herbicide resistance which makes it more difficult to control and more prone to cause yield loss. Currently waterhemp has been documented to be resistant to 6 types of herbicides. In 2011 waterhemp was found to be resistant to the most recently discovered type of herbicide. This paper investigates how the latest development of herbicide resistance is genetically controlled and passed on from generation to generation. We found that the herbicide resistance is complex and controlled by multiple genes. These findings help the weed science community have a better understanding of the genes needed for this herbicide resistance. Understanding the foundation of the resistance can lead to improved management strategies to delay or reduce the spread of this herbicide resistance.

Technical Abstract: Waterhemp (Amaranthus tuberculatus (Moq.) J.D. Sauer) is a weed prevalent in the Midwest United States and can cause yield losses up to 74% in maize (Zea mays L.) and 56% in soybean (Glycine max (L.) Merr.). An important adaptive trait commonly found in waterhemp is the ability to evolve herbicide resistance and waterhemp populations have evolved resistance to six herbicide sites of action. In 2011, two waterhemp populations were discovered resistant to p-hydroxyphenylpyruvate-dioxygenase (HPPD, EC 1.13.11.27) inhibitor herbicides. We reciprocally crossed a known HPPD-resistant waterhemp population with a known HPPD-susceptible waterhemp population and then intermated the F1 families to established a pseudo-F2 generation. We challenged the parent, F1 and pseudo-F2 generations against four HPPD-inhibiting herbicide rates (mesotrione). Our results suggest the HPPD-resistance trait is polygenic. Furthermore, the number of genes involved with the herbicide resistance increase at higher herbicide rates. These data indicated at least one dominant allele at each major locus is required to confer HPPD herbicide resistance in waterhemp. Using different waterhemp populations and methodologies, this study confirms the reported “complex” HPPD resistance inheritance while providing new information in the response of HPPD-resistant waterhemp to HPPD herbicides.