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Research Project: Monitoring and Molecular Characterization of Antimicrobial Resistance in Foodborne Bacteria

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Title: Whole genome sequencing of Escherichia coli encoding blaNDM isolated from humans and companion animals in Egypt

Author
item RAMADAN, HAZEM - Mansoura University
item SHARMA, POONAM - Orise Fellow
item GUPTA, SUSHIM - Orise Fellow
item AHMED, MARWA - Mansoura University
item Hiott, Lari
item Barrett, John
item Woodley, Tiffanie
item Frye, Jonathan
item Jackson, Charlene

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 2/22/2018
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Companion animals are a source of zoonotic infections and especially important considering the potential of companion animals to harbor antibiotic resistant pathogens. In this study, blaNDM positive Escherichia coli from companion animals, humans, and the environment from Mansoura, Egypt were characterized using Whole-genome sequencing (WGS). From December 2015 to August 2016, clinical samples (n=567) were collected from humans (n=235), companion animals (n=252), and the environment (n=80). Mid-urine samples were collected aseptically from patients admitted to Mansoura University Internal Medicine Specialized Hospital and healthy persons. Rectal (n=252) and environmental swabs (n=80) from four private animal clinics at Mansoura were included in this study. Of 165 recovered E. coli isolates, ten isolates (one each from an ill patient and healthy person, and eight from pet environment) were resistant to carbapenems phenotypically and subjected to WGS using Illumina MiSeq. Sequences were assembled using A5-miseq assembler and annotated with Prokka; ARG-ANNOT was used for detecting antibiotic resistance genes. Plasmid sequences were identified through in silico PCR identifying replicon and relaxase genes. The analysis revealed that all eight pet isolates co-harbored blaNDM-5, blaOXA-181 and blaCTX-M-15. However, some variation in resistance genes (blaNDM-1, blaOXA-1, blaCTX-M-15) and (blaNDM-5, blaOXA-1, blaCTX-M-15) were identified in isolates from the ill patient and the healthy person, respectively. All pet isolates were sequence type ST410, while the ill patient and healthy person E. coli isolates belonged to ST361 and ST167, respectively. IncF plasmids were identified in both human and pet isolates. Except for patient E. coli harboring blaNDM-1, IncX3 plasmids were detected in blaNDM-5 E. coli isolates from healthy humans and pets. These results showed that carbapenem-resistant E. coli containing identical plasmids are present in both companion animals and humans. Further characterization and genetic mapping of plasmids are required to confirm the mechanism of resistance and to determine evidence of transmission between humans and pets.