Skip to main content
ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Publications at this Location » Publication #349799
Title: Efficient transposition of the retrotransposon Tnt1 in Cucumber (Cucumis sativus L.)

Author
item ZHANG, QI - Shanghai Jiaotong University
item DU, HUI - Shanghai Jiaotong University
item LV, DUO - Shanghai Jiaotong University
item XIAO, TINGTING - Shanghai Jiaotong University
item PAN, JIAN - Shanghai Jiaotong University
item HE, HUANLE - Shanghai Jiaotong University
item WANG, GANG - Shanghai Jiaotong University
item CAI, RUN - Shanghai Jiaotong University
item Weng, Yiqun
item PAN, JUNSONG - Shanghai Jiaotong University

Submitted to: Horticultural Plant Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/1/2018
Publication Date: 3/15/2018
Citation: Zhang, Q., Du, H., Lv, D., Xiao, T., Pan, J., He, H., Wang, G., Cai, R., Weng, Y., Pan, J. 2018. Efficient transposition of the retrotransposon Tnt1 in Cucumber (Cucumis sativus L.). Horticultural Plant Journal. 4(3):111-116. https://doi.org/10.1016/j.hpj.2018.03.011.
DOI: https://doi.org/10.1016/j.hpj.2018.03.011

Interpretive Summary: Tnt1 is a type of active retrotransposon originally identified in tobacco (Nicotiana tabacum L.), but its transposition activity could be activated through tissue culture in other plant species. The insertions are stable and inheritable in the progeny, which has made it a valuable and versatile tool in developing insertional mutagenesis libraries in several plant species. Here, we explored its utility for mutagenesis in cucumber, Cucumis sativus L. T3 TNT1 transgenic cucumber plants were subjected to tissue culture to regenerate self-pollinated progeny. With PCR and analyses and Southern hybridization, we found regenerated plants maintained the original Tnt1 insertion and created new insertions suggesting characteristic re-transposition activity of Tnt1 during this process. By genome walking, some flanking sequences of Tnt1 insertions were recovered in regenerated plants. The results demonstrated that Tnt1 could be stably inherited and re-transposable during tissue culture in cucumber, and it is feasible to use for developing an insertional mutagenesis library for cucumber.

Technical Abstract: The Tnt1 is a type of active DNA retrotransposon originally identified in tobacco. The transposition activity of TNT1 could be activated through tissue culture in other plant species. The insertions of TNT1 in the recipient genome are stable and inheritable in the progeny, which has made it a valuable and versatile tool in developing insertional mutagenesis libraries in several plant species. In this study, we explored the utility of TNT1 for mutagenesis in the cucumber. T3 TNT1 transgenic cucumber plants were subjected to tissue culture to regenerate self-pollinated progeny. With PCR and analyses and Southern hybridization, we found regenerated plants maintained the original Tnt1 insertions and created new insertions suggesting characteristic re-transposition activity of Tnt1 during this process. By genome walking, some flanking sequences of Tnt1 insertions were recovered in regenerated plants. The results demonstrated that Tnt1 could be stably inherited and re-transposable during tissue culture in cucumber, and it is feasible to use for developing an insertional mutagenesis library for cucumber.