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ARS Home » Southeast Area » Dawson, Georgia » National Peanut Research Laboratory » Research » Publications at this Location » Publication #350559
Title: Population structure and association mapping to detect QTL controlling tomato spotted wilt virus resistance in cultivated peanuts

Author
item JING, LI - Auburn University
item YUEYI, TANG - Auburn University
item JACOBSON, A.L. - Auburn University
item Dang, Phat
item LI, XIAO - Auburn University
item Wang, Ming
item HAGAN, A. - Auburn University
item CHEN, C.Y. - Auburn University

Submitted to: The Crop Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/26/2018
Publication Date: 6/4/2018
Citation: Jing, L., Yueyi, T., Jacobson, A., Dang, P.M., Wang, M.L., Hagan, A., Chen, C. 2018. Population structure and association mapping to detect QTL controlling tomato spotted wilt virus resistance in cultivated peanuts. The Crop Journal. https://doi.org/10.1016/j.cj.2018.04.001.
DOI: https://doi.org/10.1016/j.cj.2018.04.001

Interpretive Summary: Spotted Wilt, caused by tomato spotted wilt virus (TSWV), is one of a major disease in cultivated peanut grown in the southeastern United States (US). Infected plants can be stunted and defoliate spotted leaves, resulting in significant yield loss. Development of resistant varieties is a sustainable strategy to combat this disease. Breeding for TSWV resistance may be facilitated by the utilization of marker assisted selection (MAS) in breeding programs to link plant resistance to the presence of specific genes. Association mapping is one type of genetic mapping that can exploit relationships between markers and traits from many lineages. The objectives of this study are to examine genetic diversity and population structure in the U.S. peanut mini core collection using simple sequence repeat (SSR)-type markers, and to conduct an association mapping of SSR markers to TSWV resistance in cultivated peanuts. One hundred and thirty-three SSR markers were used to characterize levels of resistance in 104 peanut lines from the U.S. peanut mini core collection. Four sub-groups were classified by population structure analysis which generally corresponds to the botanical varieties. Five markers were strongly associated with peanut lines with TSWV resistance. These markers can be utilized in marker-assisted selection programs to select for TSWV resistance in peanuts.

Technical Abstract: Spotted Wilt, caused by tomato spotted wilt virus (TSWV), is one of a major disease in cultivated peanut grown in the southeastern United States (US). Breeding for TSWV resistance would be facilitated by the implementation of marker assisted selection (MAS) in breeding programs, however, genes associated with resistance have not been identified. Association mapping is one type of genetic mapping that can exploit relationships between markers and traits from many lineages. The objectives of this study are to examine genetic diversity and population structure in the U.S. peanut mini core collection using simple sequence repeat (SSR) markers, and to conduct an association mapping of SSR markers to TSWV resistance in cultivated peanuts. One hundred and thirty-three SSR markers were used for genotyping the 104 genotypes from the U.S. peanut mini core collection. Four subpopulations were classified by population structure analysis which generally corresponds to botanical varieties. Association mapping analysis indicated that the five markers, pPGPseq5D5, GM1135, GM1991, TC23C08, and TC24C06, were consistently associated with TSWV resistance by population structure (Q) model, principal component analysis (PCA) model, Q+K (subpopulation) model, and PCA+K model. These identified markers could explain totally 36.4% of the phenotypic variance. Moreover, pPGPseq5D5 and GM1991 are associated with both visual symptoms of TSWV and enzyme-linked immunosorbent assay (ELISA) values with a high rate of infection. These markers can be utilized in marker-assisted selection programs to select for TSWV resistance in peanuts.