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ARS Home » Northeast Area » Washington, D.C. » National Arboretum » Floral and Nursery Plants Research » Research » Publications at this Location » Publication #350883
Research Project: Detection, Identification, and Characterization of New and Emerging Viral and Bacterial Diseases of Ornamental Plants

Location: Floral and Nursery Plants Research

Title: Host range and molecular characterization of a lytic Pradovirus-like Ralstonia phage RsPod1IDN isolated from Indonesia

Author
item ADDY, HARDIAN - University Of Jember
item FARID, MOH - University Of Jember
item EBRAHIM, ABDELMONIM ALI - Minia University
item Huang, Qi

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/18/2018
Publication Date: 9/22/2018
Citation: Addy, H.S., Farid, M.M., Ebrahim, A., Huang, Q. 2018. Host range and molecular characterization of a lytic Pradovirus-like Ralstonia phage RsPod1IDN isolated from Indonesia. Archives of Virology. 163:3409-3414. https://doi.org/10.1007/s00705-018-4033-1.
DOI: https://doi.org/10.1007/s00705-018-4033-1

Interpretive Summary: Ralstonia solanaceraum is a soil-borne and destructive phytobacterium attacking more than 450 plant species from over 50 botanical families. Control of R. solanacearum by cultural practices has not been successful due to the pathogen’s wide host range, broad distribution, great genetic variability and ability to survive in soil. ARS scientists in Beltsville, MD and collaborators from Indonesia and Egypt discovered a virus in the soil of an eggplant field in Indonesia that can kill the bacterium. They purified and characterized the virus, and determined its nucleotide and amino acid sequences. This study could lead to the development effective control for this important bacterium.

Technical Abstract: A lytic Ralstonia solanacearum-infecting phage designated RsPod1IDN was isolated from soil in Indonesia. The phage has a linear double-stranded DNA genome of 41,135 bp with 413-bp terminal repeats, and contains 41 annotated open reading frames. The phage is most closely related to Ralstonia 'RSB1, but different from 'RSB1 mainly in containing a putative HNH homing endonuclease and having a narrower host range. Our phylogenetic and genomic analyses revealed that both phage RsPod1IDN and 'RSB1 belong to the genus Pradovirus, not Phikmvvirus as previously reported for 'RSB1. RsPod1IDN is the first sequenced and characterized R. solanacearum phage isolated from Indonesia.