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ARS Home » Southeast Area » Stuttgart, Arkansas » Dale Bumpers National Rice Research Center » Research » Publications at this Location » Publication #351055
Title: Field Germplasm Evaluation, and Development of Diagnostic Methods for Bacterial Panicle Blight Disease of Rice in Arkansas

Author
item WAMISHE, YESHI - University Of Arkansas
item MULAW, TEMU - University Of Arkansas
item ROJAS, CLEMENCIA - University Of Arkansas
item Jia, Yulin
item GEBREMARIAM, TIBEBU - University Of Arkansas

Submitted to: B. R. Wells Rice Research Studies
Publication Type: Experiment Station
Publication Acceptance Date: 6/10/2018
Publication Date: 7/30/2018
Citation: Wamishe, Y., Mulaw, T., Rojas, C., Jia, Y., Gebremariam, T.A. 2018. Field Germplasm Evaluation, and Development of Diagnostic Methods for Bacterial Panicle Blight Disease of Rice in Arkansas. B. R. Wells Rice Research Studies.

Interpretive Summary:

Technical Abstract: Bacterial panicle blight (BPB), caused mainly by the bacterial pathogen Burkholderia glumae, pose a threat to rice production worldwide. Here, we report the response of over 290 rice entries evaluated by artificially inoculating with a bacterial suspension under field conditions. From the field screening of elite breeding lines in the Uniform Regional Rice Nursery (URRN), nearly 10 percent and 6 percent of the entries showed resistant and moderately resistant reaction, respectively. A subset of 10 entries showed lower BPB disease compared with other varieties in both the URRN and Arkansas Performance Trial. From 60 symptomatic samples screened on semi-selective medium, 52 were run for further molecular tests. Among these, 45 isolates were confirmed as B. glumae. The remaining seven were bacteria other than B. glumae and none of these seven isolates matched to B.gladioli. The three methods of inoculation (clip, direct injection and panicle) tested showed distinct disease phenotypes between cultivars that traditionally have been considered resistant or moderately resistant. Direct injection of bacterial inoculum into the sheath caused pathogen-related necrotic spots around the site of inoculation but not in sheaths injected with water alone. The genes Os1g32460, Os05g30500, Os11g31190, Os11g12340, Os11g12330, Os11g12040, Os11g12300, Os11g12000 and Os08g25050 were tested to determine if they are differentially expressed between the moderately resistant cultivar Jupiter and the susceptible cultivar Bengal after inoculation with B. glumae. While several of the genes were found to be upregulated after pathogen infection, none of the genes tested were differentially expressed between resistant and susceptible cultivars and the results were highly variable depending on the time of inoculation. Therefore, more optimization is needed.