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Research Project: IPM Methods for Insect Pests of Orchard Crops

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Title: Parameters on plant absortion of double-stranded Ribonucleic acid, dsRNA

Author
item CLARKE, SASHA - University Of The West Indies
item PARIS, THOMAS - University Of Florida
item Hunter, Wayne
item BROWN, SHERLINE - University Of The West Indies
item QUERESHI, JAWWAD - University Of Florida

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 2/20/2018
Publication Date: 3/9/2018
Citation: Clarke, S.V., Paris, T.M., Hunter, W.B., Brown, S.E., Quereshi, J. 2018. Parameters on plant absortion of double-stranded Ribonucleic acid, dsRNA[abstract]. 82nd Annual meeting of Florida Academy of Sciences, March 9-10,Miami, Florida. 81:4-5.

Interpretive Summary:

Technical Abstract: Efficient absorption of double-stranded Ribonucleic acid, dsRNA, into citrus is critical for effective psyllid management by RNA interference, RNAi. Parameters which might affect absorption into citrus trees and subsequent ingestion by Asian citrus psyllid were evaluated. Age of leaves, variety of citrus (rootstock versus sweet orange), and use of a surfactant, Silwet. Finally potted seedling trial conducted in a glasshouse assessed how soil treatments would affect uptake of dsRNA. Treatment of young leaves worked better than older leaves. Nymphs were significantly reduced more than adults, and soil application could deliver enough dsRNA to increase psyllid mortality and there was no difference in citrus variety noted in this trial. Results suggest that RNAi products can be delivered into citrus plants as soil applications which can reduce psyllids.