Use of omics methods to determine the mode of action of natural phytotoxins

Authors: Duke, Stephen; Pan, Zhiqiang - Peter; Bajsa-Hirschel, Joanna; SANCHEZ-MOREIRAS, ADELA - University Of Vigo; Vaughn, Justin

Submitted to: American Chemical Society Symposium Series
Publication Type: Book / Chapter
Publication Acceptance Date: 5/15/2018
Publication Date: 8/28/2018
Citation: Duke, S.O., Pan, Z., Bajsa Hirschel, J.N., Sanchez-Moreiras, A.M., Vaughn, J.N. 2018. Use of omics methods to determine the mode of action of natural phytotoxins. American Chemical Society Symposium Series. 1294:33-46.

Interpretive Summary:

Technical Abstract: Technology has greatly increased the power of omics methods to profile transcription, protein, and metabolite responses to phytotoxins. These methods hold promise as a tool for providing clues to the modes of action of such compounds. However, to date, only two putative modes of action have been found with these methods; one with proteomics and the other with metabolomics. As with traditional physiological methods (physionomics) for mode of action discovery, differentiating between primary and secondary and tertiary effects is problematic. This problem can partially be overcome by careful experimental design. More powerful tools for metabolic pathway analysis are making transcriptome data easier to interpret with regard to the potential phytotoxin target site. Stable isotope measurement of metabolite pool turnover (fluxomics) has the potential to improve the insight into metabolomics for mode of action discovery.