Author
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CANO, L.M - University Of Florida |
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OPPELAAR, T.S - University Of Florida |
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RODRIGUES, L.N - University Of Florida |
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CHAULAGAIN, B - University Of Florida |
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HINCAPIE, M - University Of Florida |
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Sood, Sushma |
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ZHENG, Q - University Of Florida |
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COMSTOCK, JACK - Former ARS Employee |
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URASHIMA, SEITI - Sao Paulo State University (UNESP) |
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ROTT, PHILIPE - University Of Florida |
Submitted to: American Society of Sugar Cane Technologists
Publication Type: Abstract Only Publication Acceptance Date: 4/30/2018 Publication Date: N/A Citation: N/A Interpretive Summary: N/A Technical Abstract: Puccinia kuehnii, the causal agent of orange rust, is a fungal pathogen that causes damaging losses to the sugarcane crop worldwide. Rust fungi deliver effector proteins into infected host tissues using a specialized feeding structure called haustorium, to manipulate plant functions and promote parasitic growth. Effector protein repertoires have been characterized in several Puccinia species and other devastating rust fungi. In this study, the genomes of two Florida isolates of P. kuehnii (1040 and 2143) varying in virulence were sequenced. Draft whole-genome and transcriptome assemblies from isolates 1040 and 2143 were used to perform sequence comparisons (BLASTn) against a list of 40 haustoria expressed effector genes from various rust fungi. These sequence comparisons allowed us to identify homolog gene sequences in P. kuehnii genomes to known suppressors of hypersensitive response (SHR) effector proteins. These homologs will be used to characterize genome sequence variations and to identify molecular makers that could be associated with virulence in the sugarcane orange rust pathogen. Our findings are a first step toward improving sugarcane rust disease management using next-generation technologies. |