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ARS Home » Plains Area » Kerrville, Texas » Knipling-Bushland U.S. Livestock Insects Research Laboratory » LAPRU » Research » Publications at this Location » Publication #352801
Title: Transcriptome sequencing of newly molted adult female cattle ticks, Rhipicephalus microplus: Raw Illumina reads.

Author
item Guerrero, Felicito
item Bendele, Kylie

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: 2/23/2018
Publication Date: 2/11/2019
Citation: Guerrero, F., Bendele, K.G. 2018. Transcriptome sequencing of newly molted adult female cattle ticks, Rhipicephalus microplus: Raw Illumina reads.. National Center for Biotechnology Information (NCBI). GenBank Accession sequence submissions PRJNA431211, SAMN08405855-SAMN08405857, SRR6765804-SRR6765808.

Interpretive Summary: Next-generation DNA sequencing technology was used to sequence the transcriptome from newly molted adult females from the cattle tick, Rhipicephalus microplus. These samples include newly molted unfed whole adult females, newly molted whole adult females feeding for 2 hours on a bovine host, and newly molted unfed whole adult females enclosed within a gas permeable bag attached to the side of a bovine host such that the ticks could sense the animal but could not feed. Each corresponding dataset of raw sequence reads has been deposited at NCBI under the BioProject accession number PRJNA431211 and and BioSample accession numbers SAMN08405855-SAMN08405857, respectively. The Short Read Archive (SRA) accession numbers are SRR6765804-SRR6765808. USDA is an equal opportunity employer.

Technical Abstract: Illumina paired end oligo-dT sequencing technology was used to sequence the transcriptome from newly molted adult females from the cattle tick, Rhipicephalus microplus. These samples include newly molted unfed whole adult females, newly molted whole adult females feeding for 2 hours on a bovine host, and newly molted unfed whole adult females enclosed within a gas permeable bag attached to the side of a bovine host such that the ticks could sense the animal but could not feed. Each corresponding dataset of raw sequence reads has been deposited at NCBI under the BioProject accession number PRJNA431211 and and BioSample accession numbers SAMN08405855-SAMN08405857, respectively. The Short Read Archive (SRA) accession numbers are SRR6765804-SRR6765808. USDA is an equal opportunity employer.