Skip to main content
ARS Home » Plains Area » Lincoln, Nebraska » Wheat, Sorghum and Forage Research » Research » Publications at this Location » Publication #353610
Research Project: Genetic Improvement of Sorghum for Bioenergy, Feed, and Food Uses

Location: Wheat, Sorghum and Forage Research

Title: Tri5 gene expression analysis and postharvest accumulation of deoxynivalenol during storage of hard red winter wheat

Author
item BOLANOS-CARRIEL, CARLOS - University Of Nebraska
item HALLEN-ADAMS, HEATHER - University Of Nebraska
item WEGULO, STEPHEN - University Of Nebraska
item BAENZIGER, STEPHEN - University Of Nebraska
item ESKRIDGE, KENT - University Of Nebraska
item Funnell-Harris, Deanna
item MCMASTER, NIKI - Virginia Tech
item SCHMALE, DAVID - Virginia Tech

Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 6/12/2018
Publication Date: 6/12/2018
Citation: Bolanos-Carriel, C., Hallen-Adams, H., Wegulo, S.N., Baenziger, S.P., Eskridge, K.M., Funnell-Harris, D.L., Mcmaster, N., Schmale, D. 2018. Tri5 gene expression analysis and postharvest accumulation of deoxynivalenol during storage of hard red winter wheat. American Phytopathological Society Abstracts. [asbstract].In:Proceedings of 2018 American Phytopathological Society North Central Division Meeting, June 12-14, 2018 Fargo ND.

Interpretive Summary:

Technical Abstract: Wheat cultivation is vital to ensuring food security around the world. The crop is severely affected by disease problems such as scab caused by Fusarium graminearum. The fungus produces harmful mycotoxins such as deoxynivalenol (DON). Postharvest storage experiments were conducted using grain samples from field plots treated with a strobilurin fungicide, a triazole fungicide, or left untreated. Grain samples were brought to 20% moisture and stored for 120 days. DON accumulation and trichodiene synthase gene (Tri5) expression were monitored using GC-MS and qRT-PCR, respectively. Tri5 gene expression was normalized using the housekeeping gene GAPDH. Cultivar resistance to scab affected both the detection of Tri5 and DON concentration. The triazole treatment consistently showed lower levels of DON than the strobilurin treatment and the unsprayed checks irrespective of the cultivar. Furthermore, only in the triazole treatment, a reduction in the relative expression of the Tri5 was detected after 120 days in the scab-susceptible cultivar. In the scab-susceptible cultivar, after 120 days, DON levels increased significantly in the strobilurin treatment (from 2.86 to 3.49 ppm), while relative Tri5 gene expression also increased significantly (0.73 to 0.80) in the non-fungicide treated checks. Our study demonstrates that the genetic expression of the Tri5 that is necessary for the production of DON can be increased during storage of high-moisture grain.