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ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Publications at this Location » Publication #353633
Research Project: Maximizing the Impact of Potato Genebank Resources: Development and Evaluation of a Wild Species Genotype Diversity Panel

Location: Vegetable Crops Research

Title: Genetic evidence of a lethal recessive allele on chromosome 12 of potato

Author
item ENDELMAN, JEFFREY - UNIVERSITY OF WISCONSIN
item JANSKY, SHELLEY
item BUTLER, NATHAN - UNIVERSITY OF WISCONSIN

Submitted to: Potato Association of America Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 3/19/2018
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: n 1998, Hosaka and Hanneman reported an S-locus inhibitor (Sli) locus linked to a lethal recessive allele at the end of chromosome 12. This hypothesis served to explain their inability to fix self-compatibility in a highly inbred line of S. chacoense and distorted segregation ratios for the self-compatible phenotype and linked genetic markers in a diploid mapping population. We have detected a lethal recessive allele at the end of chromosome 12 in two F2 mapping populations, inferred based on the complete absence of one homozygous genotype. The grandparents of the first F2 population are DM1-3, the doubled monoploid used for the potato reference genome, and M6, a highly inbred and self-compatible S. chacoense clone. Over 300 progeny have been genotyped in this population, using both the potato SNP array and sequence-based genotyping (SBG) of restriction-site associated DNA, and no progeny are homozygous for the DM1-3 allele in the interval 57.2–57.8 Mb on chromosome 12. The grandparents of the second F2 population are US-W4, a self-compatible primary dihaploid derived from the University of Minnesota breeding program, and S. chacoense 39-7, a highly inbred, self-compatible clone. This population exhibits a recombination hotspot at the end of chromosome 12, with 12 of 23 progeny genotyped with the potato SNP array having a recombination event between 57.6 and 59.2 Mb. Furthermore, none of the progeny (including several that are self-compatible) are homozygous for the 39-7 allele in this interval. The results suggest that both DM1-3 and 39-7 contain the lethal recessive allele observed by Hosaka and Hanneman. Since DM1-3 is derived from anther culture, we hypothesize the allele is lethal during seed development or germination.