Location: Nat'l Clonal Germplasm Rep - Tree Fruit & Nut Crops & Grapes
Title: DNA profiling of figs (Ficus carica L.) from Slovenia and Californian USDA collection revealed the uniqueness of some North Adriatic varietiesAuthor
KNAP, TEA - University Of Primorska | |
Aradhya, Mallikarjuna | |
ARBEITER, ALENKA BARUCA - University Of Primorska | |
HLADNIK, MATJAZ - University Of Primorska | |
BANDELJ, DUNJA - University Of Primorska |
Submitted to: Genetic Resources and Crop Evolution
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 3/26/2018 Publication Date: 4/7/2018 Citation: Knap, T., Aradhya, M.K., Arbeiter, A., Hladnik, M., Bandelj, D. 2018. DNA profiling of figs (Ficus carica L.) from Slovenia and Californian USDA collection revealed the uniqueness of some North Adriatic varieties. Genetic Resources and Crop Evolution. 65:1503-1516. https://doi.org/10.1007/s10722-018-0634-5. DOI: https://doi.org/10.1007/s10722-018-0634-5 Interpretive Summary: A set of 23 local varieties from Slovenia and 218 fig accessions from Californian fig germplasm collection were compared to determine the identity of genotypes and their possible genetic relationships. Figs were genotyped using twelve microsatellite loci. One hundred alleles were identified over all microsatellite loci with an average of 8.33 alleles per locus and a polymorphic information content of 0.557 per locus. DNA genotyping demonstrated a relatively high level of genetic diversity between analysed figs. Comparison of fig genotypes from Slovenia and California demonstrated that only six Slovenian varieties shared identical DNA profiles with figs from the Californian collection, while the other 17 Slovenian varieties were unique and characteristic to the North Adriatic region. The information obtained will contribute to a better management of fig genetic resources. Technical Abstract: A set of 23 local varieties from Slovenia and 218 fig accessions from Californian fig germplasm collection were compared to determine the identity of genotypes and their possible genetic relationships. Figs were genotyped using twelve microsatellite loci. One hundred alleles were identified over all microsatellite loci with an average of 8.33 alleles per locus and a polymorphic information content of 0.557 per locus. DNA genotyping demonstrated a relatively high level of genetic diversity between analysed figs. Comparison of fig genotypes from Slovenia and California demonstrated that only six Slovenian varieties shared identical DNA profiles with figs from the Californian collection, while the other 17 Slovenian varieties were unique and characteristic to the North Adriatic region. The information obtained will contribute to a better management of fig genetic resources. |