Complete genome sequence of multidrug-resistant plesiomonas shigelloides strain MS-17-188

Authors: ABDELHAMED, HOSSAM - Mississippi State University; OZDEMIR, OZAN - Mississippi State University; TEKEDAR, HASAN - Mississippi State University; ARICK, MARK II - Mississippi State University; HSU, CHUAN-YU - Mississippi State University; KARSI, ATTILA - Mississippi State University; LAWRENCE, MARK - Mississippi State University

Submitted to: Genome Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/2/2018
Publication Date: 5/3/2018
Citation: Abdelhamed, H., Ozdemir, O., Tekedar, H., Arick, M., Hsu, C., Karsi, A., Lawrence, M. 2018. Complete genome sequence of multidrug-resistant plesiomonas shigelloides strain MS-17-188. Genome Announcements. http://doi.org/10.1128/genomeA/00387-18.
DOI: https://doi.org/10.1128/genomeA/00387-18

Interpretive Summary: Plesiomonas shigelloides is the predominant species isolated from intestinal microflora of catfish, catfish pond sediment, and water in the southeastern United States. Plesiomonas shigelloides is a Gram-negative bacterium isolated from diverse environments. In this work, we described the complete genomic sequence of the multidrug-resistant P. shigelloides strain MS-17-188 isolated from a diseased catfish, which contains a circular chromosome of 3,970,359 bp and three plasmids, which carry antibiotic genes. Understanding these genes is important for designing strategies for eliminating this fish pathogen.

Technical Abstract: Plesiomonas shigelloides is the predominant species isolated from intestinal microflora of catfish, catfish pond sediment, and water in the southeastern United States. P. shigelloides strain MS-17-188 was recovered from a diseased catfish in 2017 from the Aquatic Diagnostic Laboratory at the College of Veterinary Medicine, Mississippi State University. P. shigelloides strain MS-17-188 is resistant to gentamicin, tetracycline, oxytetracycline, doxycycline, sulfamethoxazole-trimethoprim, florfenicol, erythromycin, chloramphenicol, streptomycin, azithromycin, penicillin, novobiocin, and spectinomycin. A team of scientists from Mississippi State University used Illumina and Nanopore to produce genome sequence assembly of the of MS-17-188 stain. The complete genome sequence of P. shigelloides MS-17-188 comprised of a single chromosome and three circular plasmids (pPS-MS-17-188-1, pPS-MS-17-188-2, and pPS-MS-17-188-3). Among the three plasmids, the pPS-MS-17-188-1 and pPS-MS-17-188-3 plasmids were found to carry several antibiotic resistance genes.