Location: Warmwater Aquaculture Research Unit
Title: Small molecules targeting LapB protein prevent Listeria attachment to catfish muscleAuthor
AKGUL, ALI - Mississippi State University | |
AL-JANABI, NAWAR - Mississippi State University | |
DAS, BHASKAR - Mount Sinai School Of Medicine | |
LAWRENCE, MARK - Mississippi State University | |
KARSI, ATTILA - Mississippi State University |
Submitted to: PLOS ONE
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 12/1/2017 Publication Date: 12/18/2018 Citation: Akgul, A., Al-Janabi, N., Das, B., Lawrence, M., Karsi, A. 2018. Small molecules targeting LapB protein prevent Listeria attachment to catfish muscle. PLoS One. 12(12):1-10. Interpretive Summary: Listeria monocytogenes is an important food-borne pathogen causing listeriosis in human and animals. Relatively little is known about the attachment mechanisms of Listeria to channel catfish fillets. The goal of the present study was to determine the potential role of Listeria LapB protein in catfish fillet attachment. To achieve this, boron-based small molecules were designed to interfere with the active site of LapB protein. Results indicated that majority of small molecules were effective in reducing the Listeria attachment to catfish fillets. Technical Abstract: Listeria monocytogenes is a Gram-positive foodborne pathogen and the causative agent of listeriosis. L. monocytogenes lapB gene encodes a cell wall surface anchor protein, and mutation of this gene causes Listeria attenuation in mice. In this work, the potential role of Listeria LapB protein in catfish fillet attachment was investigated. To achieve this, boron-based small molecules designed to interfere with the active site of the L. monocytogenes LapB protein were developed, and their ability to prevent L. monocytogenes attachment to fish fillet was tested. Results indicated that seven out of nine different small molecules were effective in reducing the Listeria attachment to catfish fillets. Of these, three small molecules (SM3, SM5, and SM7) were highly effective in blocking Listeria attachment to catfish fillets. This study suggests an alternative strategy for reduction of L. monocytogenes contamination in fresh and frozen fish products. |