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ARS Home » Southeast Area » Raleigh, North Carolina » Soybean and Nitrogen Fixation Research » Research » Publications at this Location » Publication #355112
Research Project: Exploiting Genetic Diversity through Genomics, Plant Physiology, and Plant Breeding to Increase Competitiveness of U.S. Soybeans in Global Markets

Location: Soybean and Nitrogen Fixation Research

Title: Effect of a mutant Danbaekkong allele on soybean seed yield, protein, and oil concentration and amino acid composition

Author
item CUNICELLI, MIA - University Of Tennessee
item BHANDARI, HEM - University Of Tennessee
item CHEN, PENGYIN - University Of Missouri
item SAMS, CARL - University Of Tennessee
item Mian, Rouf
item MOZZONI, LEANDRO - University Of Arkansas
item SMALLWOOD, CHRISTOPHER - University Of Tennessee
item PANTALONE, VINCENT - University Of Tennessee

Submitted to: Journal of the American Oil Chemists' Society
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/9/2019
Publication Date: 7/9/2019
Citation: Cunicelli, M.J., Bhandari, H.S., Chen, P., Sams, C.E., Mian, R.M., Mozzoni, L.A., Smallwood, C.J., Pantalone, V.R. 2019. Effect of a mutant Danbaekkong allele on soybean seed yield, protein, and oil concentration and amino acid composition. Journal of the American Oil Chemists' Society. https://doi.org/10.1002/aocs.12261.
DOI: https://doi.org/10.1002/aocs.12261

Interpretive Summary: The negative correlation between soybean [Glycine max (L.) Merr.] seed protein and oil and between protein and yield has hampered the development of high protein soybean cultivars with high oil and high yield. A major high-protein allele from Korean cultivar Danbaekkong is known to increase seed protein by 3-4%, but its effect on seed yield is controversial. The objective of this study was to determine if marker assisted selection for the Danbaekkong (Dan) protein allele on chromosome 20 influences agronomic traits in selected genetic backgrounds. A population of 24 F8:10 near isogenic lines (NIL) of soybean was created by crossing G03-3101 × LD00-2817P. The 24 NIL consisted of 12 wild type (WT) and 12 mutant Dan type lines. These NIL were grown in 2016 and 2017 field seasons in replicated field trials in nine environments, with six in Tennessee and one each in Arkansas, Missouri, and North Carolina. There were significant (p < 0.05) differences in yield, protein, and oil concentrations between the two experimental groups. The Dan group had significantly (p < 0.05) more protein (421 g kg-1), less oil (192 g kg-1), and lower yield (3143 kg ha-1) than the WT group (390 g kg-1 protein, 210 g kg-1 oil, 3281 kg ha-1 yield). These results support previous research and corroborate the overall negative genetic correlations. Seed yield of several higher protein Dan lines MC-13, MC-16, MC-19, and MC-24, however, exceeded seed yield of lower protein WT lines MC-2, MC-3, MC-6, and MC-10 and the formers represent genetic resources for reducing the negative correlation between protein and yield. This study also demonstrates that seed protein and seed yield in soybean are not pleiotropic.

Technical Abstract: There is a known negative correlation between soybean [Glycine max (L.) Merr.] seed protein and oil and between protein and yield, challenging breeders to increase protein concentration while maintaining oil concentration and yield. The objective of this study was to determine if marker assisted selection for the Danbaekkong (Dan) protein allele on chromosome 20 influences quality traits in selected genetic backgrounds. A population of 24 F8:10 near isogenic lines (NIL) of soybean was created by crossing G03-3101 × LD00-2817P. The 24 NIL consisted of 12 wild type (WT) and 12 mutant Dan type lines. These NIL were grown in 2016 and 2017 field seasons in replicated field trials in nine environments, with six in Tennessee and one each in Arkansas, Missouri, and North Carolina. There were significant (p < 0.05) differences in yield, protein, and oil concentrations between the two experimental groups. The Dan group had significantly (p < 0.05) more protein (421 g kg-1), less oil (192 g kg-1), and lower yield (3143 kg ha-1) than the WT group (390 g kg-1 protein, 210 g kg-1 oil, 3281 kg ha-1 yield). These results support previous research and corroborate the overall negative genetic correlations. Seed yield of several higher protein Dan lines MC-13, MC-16, MC-19, and MC-24 exceeded seed yield of lower protein WT lines MC-2, MC-3, MC-6, and MC-10 and the formers represent genetic resources for reducing the negative correlation between protein and yield.