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ARS Home » Southeast Area » Little Rock, Arkansas » Arkansas Children's Nutrition Center » Research » Publications at this Location » Publication #355186
Title: Functional differences in platelet mitochondrial respiration in children with excess weight

Author
item DIAZ, EVA - University Arkansas For Medical Sciences (UAMS)
item WEBER, JUDITH - Arkansas Children'S Hospital
item Ferruzzi, Mario
item COTTER, MATTHEW - Arkansas Children'S Hospital
item HENRY, AUSTIN - Arkansas Children'S Hospital
item YOUNG, CATARINA - Arkansas Children'S Hospital
item BORSHEIM, ELISABET - Arkansas Children'S Nutrition Research Center (ACNC)

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/17/2018
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Metabolic stress modulates mitochondrial energetics of circulating cells. The purpose of this preliminary study was to characterize the mitochondrial respirometric profile of permeabilized platelets in school-age children with and without excess weight (EW). Eleven normal weight (NW) and 26 with EW (BMI>85th percentile) 9-year-old children were studied. Mitochondrial function of permeabilized platelets was assessed using high-resolution respirometry. Routine respiration (R), Complex (C)I-leak (L, pyruvate & malate), OXPHOS capacity of CI-linked activity (P, ADP), maximal respiration (E, CCCP as chemical uncoupler), uncoupled CI-respiration (CIE, glutamate), uncoupled CI&CII respiration (CIE&IIE, succinate), CII-linked ETS-capacity (CIIE, rotenone), residual oxygen consumption (ROX, antimycin), and CIV activity (TMPD) were measured. Flux control ratios (FCR) were calculated by dividing fluxes (pmol/s-1/10-6) in all respiratory states by CIE&IIE. OXPHOS coupling efficiency (j~p=1-L/P), Free electron transport capacity (~E=E-L), Excess capacity factor (JExP=1-P/E), and spare respiratory capacity (SRC=E-R) were calculated. Data presented as means +/- SD. Children differed in BMI percentile (EW=93+/-5 vs. NW=53+/-23; p<.0001). FCR's and j~P were not different between groups. However, ~E, JExP, and SRC were higher in the EW group (~E: EW=0.29+/-0.08 vs. NW=0.22+/-0.06; p=0.038; JExP: EW= 0.36+/-0.07 vs. NW=0.28+/-0.09; p=0.029; SRC: EW=0.26+/-0.10 vs. NW=0.19+/-0.06; p=0.040). Permeabilized platelets of children with EW exhibit increased respiratory capacity potential (~E), increased control of the phosphorylation system over OXPHOS capacity (JExP) and increased SRC. This may indicate greater mitochondrial abundance in platelets from children with EW. The translational aspect and time course of these adaptations warrant further investigation.