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ARS Home » Northeast Area » Beltsville, Maryland (BHNRC) » Beltsville Human Nutrition Research Center » Diet, Genomics and Immunology Laboratory » Research » Publications at this Location » Publication #355188
Title: Suppression of T lymphocyte activation by 3-chloro-1,2-propanediol mono- and di-palmitate esters in vitro

Author
item HUANG, G - Shanghai Jiaotong University
item XUE, J - Shanghai Jiaotong University
item SUN, X - Shanghai Jiaotong University
item WANG, J - Shanghai Jiaotong University
item WANG, Y - Fudan University
item Wang, Thomas - Tom
item YU, LIANGLI - University Of Maryland

Submitted to: Toxicology In Vitro
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/3/2018
Publication Date: 9/1/2018
Citation: Huang, G., Xue, J., Sun, X., Wang, J., Wang, Y., Wang, T.T., Yu, L. 2018. Suppression of T lymphocyte activation by 3-chloro-1,2-propanediol mono- and di-palmitate esters in vitro. Toxicology In Vitro. 51:54-62. https://doi.org/10.1016/j.tiv.2018.05.002.
DOI: https://doi.org/10.1016/j.tiv.2018.05.002

Interpretive Summary: Food contaminants may effect human health. This study investigated whether and how 3-chloro-1,2-propanediol (3-MCPD) fatty acid esters, a group of food contaminants formed during processing, might inhibit the immune system through suppressing T lymphocyte activation for the first time. Three 3-MCPD esters including 1-palmitoyl-3-chloropropanediol (1-pal), 2-palmitoyl-3-chloropropanediol (2-pal), and1,2-dipalmitoyl-3-chloropropanediol (dipal) were selected as the probe compounds to test the possible effects of fatty acid structure on their potential immune inhibitory effect. The results showed that 1-pal and 2-pal, but not dipal, significantly suppressed ConA-induced T lymphocyte proliferation, cell cycle activity, Th1 and Th2 cytokine secretion, CD4+ T cell populations, and the ratio of CD4+/CD8+ T cells under the experimental conditions. Moreover, Western blotting and immunofluorescence analyses revealed that 1-pal and 2-pal could inhibit the activation of ConA-stimulated mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-'B) signaling pathways. In addition, 1-pal significantly suppressed DNFB-induced delayed-type hyper sensitivity (DTH) reaction characterized by the increased ear thickness and IFN-' production in mice. These observations indicated that 3-MCPD esters exerted a negative effect on T lymphocyte-mediated immunity, and the immunosuppressive activities of 3-MCPD monopalmitates were stronger than 3-MCPD dipalmitate.

Technical Abstract: This study investigated whether and how 3-chloro-1,2-propanediol (3-MCPD) fatty acid esters, a group of food contaminants formed during processing, might inhibit the immune system through suppressing T lymphocyte activation for the first time. Three 3-MCPD esters including 1-palmitoyl-3-chloropropanediol (1-pal), 2-palmitoyl-3-chloropropanediol (2-pal), and1,2-dipalmitoyl-3-chloropropanediol (dipal) were selected as the probe compounds to test the possible effects of fatty acid structure on their potential immune inhibitory effect. The results showed that 1-pal and 2-pal, but not dipal, significantly suppressed ConA-induced T lymphocyte proliferation, cell cycle activity, Th1 and Th2 cytokine secretion, CD4+ T cell populations, and the ratio of CD4+/CD8+ T cells under the experimental conditions. Moreover, Western blotting and immunofluorescence analyses revealed that 1-pal and 2-pal could inhibit the activation of ConA-stimulated mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-'B) signaling pathways. In addition, 1-pal significantly suppressed DNFB-induced delayed-type hyper sensitivity (DTH) reaction characterized by the increased ear thickness and IFN-' production in mice. These observations indicated that 3-MCPD esters exerted a negative effect on T lymphocyte-mediated immunity, and the immunosuppressive activities of 3-MCPD monopalmitates were stronger than 3-MCPD dipalmitate.