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ARS Home » Northeast Area » Leetown, West Virginia » Cool and Cold Water Aquaculture Research » Research » Publications at this Location » Publication #355451
Research Project: Integrated Research to Improve On-Farm Animal Health in Salmonid Aquaculture

Location: Cool and Cold Water Aquaculture Research

Title: Proteome analysis reveals a role of rainbow trout lymphoid organs during the Yersinia ruckeri infection process

Author
item KUMAR, GOKHLESH - University Of Veterinary Medicine
item HUMMEL, KARIN - University Of Veterinary Medicine
item NOEBAUER, KATHARINA - University Of Veterinary Medicine
item Welch, Timothy - Tim
item RAZZAZI-FAZELI, EBRAHIM - University Of Veterinary Medicine
item EL-MATBOUL, MANSOUR - University Of Veterinary Medicine

Submitted to: Scientific Reports
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/30/2018
Publication Date: 9/18/2019
Citation: Kumar, G., Hummel, K., Noebauer, K., Welch, T.J., Razzazi-Fazeli, E., El-Matboul, M. 2019. Proteome analysis reveals a role of rainbow trout lymphoid organs during the Yersinia ruckeri infection process. Scientific Reports. 8:13998. https://doi.org/10.1038/s41598-018-31982-6.
DOI: https://doi.org/10.1038/s41598-018-31982-6

Interpretive Summary: Enteric redmouth disease (ERM) causes significant economic losses in salmonid aquaculture worldwide. The disease is caused by Yersinia ruckeri, a Gram negative rod-shaped enterobacterium. The aim of this study was to identify and quantify rainbow trout lymphoid organ proteomic expression profiles in response to infection with biotype 1 and biotype 2 Y. ruckeri strains by SWATH-MS, a label-free quantitative proteomic approach. This work identified 34 proteins from head kidney and 85 proteins from spleen that were differentially expressed during Y. ruckeri infection. The proteins identified were predicted to function in immune response, antioxidant activities, cytoskeleton, transport, ion binding, and metabolic processes. This information adds to our basic understanding of the host-pathogen interactions that lead to disease.

Technical Abstract: Yersinia ruckeri is the causative agent of enteric redmouth disease in salmonids. Head kidney and spleen are major lymphoid organs of the teleost fish where antigen presentation and immune defense against microbes take place. We investigated proteome alteration in head kidney and spleen of the rainbow trout (Oncorhynchus mykiss) following Y. ruckeri CSF007-82 and 7959-11 strains infection. Each of these organs were analyzed after 3, 9 and 28 days post exposure with a shotgun proteomic approach using SWATH, a label-free quantitative approach. Sophisticated statistical analyses were used to identify proteome differences among infected organs at different time points. GO annotation and protein-protein interaction were predicted using bioinformatic tools. Thirty four proteins from head kidney and 85 proteins from spleen were found to be differentially expressed in rainbow trout during the Y. ruckeri infection process. These proteins mostly are related to immune response, antioxidant, cytoskeleton, transport, ion binding, and metabolic processes. The findings of this study regarding the immune response at the protein level offer new insight into the systemic response to Y. ruckeri infection in rainbow trout. This proteomic data facilitate a better understanding of host-pathogen interactions and response of fish against Y. ruckeri biotype 1 and 2 strains.