Registration of a bread wheat recombinant inbred line mapping population derived from a cross between 'Harry' and 'Wesley'

Authors: HUSSAIN, WASEEM - University Of Nebraska; Guttieri, Mary; BELAMKAR, VIKAS - University Of Nebraska; POLAND, JESSE - Kansas State University; SALLAM, AHMED - Assiut University; BAENZIGER, P.STEPHEN - University Of Nebraska

Submitted to: Journal of Plant Registrations
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/5/2018
Publication Date: 7/2/2018
Citation: Hussain, W., Guttieri, M.J., Belamkar, V., Poland, J., Sallam, A., Baenziger, P. 2018. Registration of a bread wheat recombinant inbred line mapping population derived from a cross between 'Harry' and 'Wesley'. Journal of Plant Registrations. https://doi.org/10.3198/jpr2017.11.0085crmp.
DOI: https://doi.org/10.3198/jpr2017.11.0085crmp

Interpretive Summary: Breeding wheat for tolerance to drought stress is challenging because the underlying genetics that control drought tolerance are not yet well understood in wheat. A set of true-breeding offspring of a cross between two hard red winter wheat varieties, 'Harry’ and ‘Wesley’, was developed to provide a resource for subsequent experiments to better understand drought tolerance in winter wheat. A wide range of plant characteristics were observed among these offspring. DNA sequence variants were identified among the offspring using the genotyping-by-sequencing approach, and a high-density map of these variants in the wheat genome was created. The quality and accuracy of the map was confirmed by mapping two known traits, chaff color and leaf waxiness, which is sometimes associated with tolerance to drought stress. This important germplasm resource was deposited in the National Small Grains Collection, and together with the DNA sequence variant information, provides the community of wheat researchers with access to a diverse set of lines in combination with a high-density genetic map that can be used to further characterize the genes associated with drought tolerance and wide adaptation in the US Great Plains.

Technical Abstract: The hard red winter wheat (Triticum aestivum L.) ‘Harry’ x ‘Wesley’ mapping population (Reg. No. MP-9, NSL 523949 MAP) consists of 203 recombinant inbred lines and was developed at the University of Nebraska, Lincoln. The mapping population (F6:11) was derived from a cross between Harry (drought tolerant) and Wesley (drought susceptible)wheat cultivars using the single-seed descent method. The primary goal in developing this mapping population was to identify genomic regions associated with agronomic traits in response to drought in the Harry and Wesley cultivars. The population segregates for a number of traits, including chaff color, leaf waxiness, flowering date, plant height, grain yield, yield component traits, and resistance to tan spot. A wide range of phenotypic diversity among individuals and transgressive segregation were observed for all segregating traits. The mapping population was genotyped using the genotyping-by-sequencing approach, and a high-density linkage map developed. The quality and accuracy of the linkage map have been confirmed by mapping known genomic regions for two highly heritable traits, chaff color and leaf cuticular wax. The mapping population is an important germplasm resource that will provide wheat breeders with a diverse set of lines in combination with a high-density genetic map that can be utilized to identify genomic regions at high mapping resolution and breed for drought tolerance and wider adaptation in the US Great Plains.