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ARS Home » Midwest Area » Ames, Iowa » National Laboratory for Agriculture and The Environment » Agroecosystems Management Research » Research » Publications at this Location » Publication #356057
Research Project: Agroecosystem Benefits from the Development and Application of New Management Technologies in Agricultural Watersheds

Location: Agroecosystems Management Research

Title: Temporal dynamics of bacterial communities in soil, and leachate water after swine manure application

Author
item RIEKE, ELIZABETH - Iowa State University
item Moorman, Thomas
item SOUPIR, MICHELLE - Iowa State University
item HOWE, ADINA - Iowa State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/30/2018
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Land application of swine manure recycles plant nutrients, but excess nutrients and zoonotic bacteria may degrade water quality especially where fields have subsurface drainage. We investigated the temporal impact of swine manure application on soil microbial communities. Swine manure was applied to intact soil columns collected from plots under chisel plow or no-till with corn and soybean rotation. In the following 108 days, six simulated rainfalls were applied and drainage water was collected. DNA extraction and targeted 16S-rRNA gene sequencing was used to characterize and to identify shifts in bacterial communities in the soil and drainage water over 108 days after manure application. Tillage accounted for only a small fraction of the variability in bacterial communities. The majority of OTUs stimulated by manure application belonged to 12 orders of bacteria. Orders within the Proteobacteria were most prevalent, followed by Bacteroidetes, Firmicutes, Actinobacteria and Spirochaetes. While the majority of the 12 orders decreased after day 59, relative abundances of OTU associated with Rhizobiales and Actinomyecetales in soil continued to increase. Bacterial orders which were stimulated by manure application in soil had varied responses in drainage waters over the course of the experiment. We identified a “manure-specific core” of five genera who comprised 13% of manure community. Of these five genera, Clostridium sensu stricto was the only genus which did not return to pre-manure relative abundance in soil by day 108. Our results show that enrichment responses after manure amendment could result from displacement of autochthonous soil bacteria by manure-borne bacteria during the application process or by growth of indigenous bacteria using manure-derived nutrients.