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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Publications at this Location » Publication #356330
Title: High-density linkage map construction and mapping QTLs for yield and yield components in autotetraploid alfalfa using RAD-seq

Author
item ZHANG, FAN - Chinese Academy Of Agricultural Sciences
item KANG, JUNMEI - Chinese Academy Of Agricultural Sciences
item LONG, RUI-CAI - Chinese Academy Of Agricultural Sciences
item Yu, Long-Xi
item WANG, ZHEN - Chinese Academy Of Agricultural Sciences
item ZHAO, ZHONGXIANG - Chinese Academy Of Agricultural Sciences
item ZHANG, TIEJUN - Chinese Academy Of Agricultural Sciences
item YANG, QINGCHUAN - Chinese Academy Of Agricultural Sciences

Submitted to: BMC Plant Biology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/10/2019
Publication Date: 4/27/2019
Citation: Zhang, F., Kang, J., Long, R., Yu, L., Wang, Z., Zhao, Z., Zhang, T., Yang, Q. 2019. High-density linkage map construction and mapping QTLs for yield and yield components in autotetraploid alfalfa using RAD-seq. Biomed Central (BMC) Plant Biology. 19(165):1-12. https://doi.org/10.1186/s12870-019-1770-6.
DOI: https://doi.org/10.1186/s12870-019-1770-6

Interpretive Summary: Alfalfa (Medicago sativa L.) is an important legume forage grown worldwide. Alfalfa is called “Queen of Forage” due to its high production and nutrition values.Tthe present study reports the identification of quantitative trait loci (QTL) associated with biomass yield and yield component in a biperental population of alfalfa. QTLs for yield, plant height, shoot diameter and branch number have been mapped in a high density genetic map.Among them, some QTLs were consistently identified in multiple traits and co-located . With further validation, these QTLs and closely linked markers can be used for maker -assisted selection (MAS) in breeding programs to improved alfalfa yield.

Technical Abstract: In the present study, a high-density linkage map was constructed using single nucleotide polymorphism (SNP) markers using restriction-associated DNA sequencing (RAD-seq). The linkage map contains 4346 SNP and 119 SSR markers, with 64 linkage groups across two parents. The average marker distance is 3.00 and 1.32 cM for paternal and maternal linkage map. Using this high-density linkage map, we mapped QTLs for yield, plant height, shoot diameter and branch number.Among them, some QTLs were consistently identified in multiple traits and co-located . With further validation, these QTLs and closely linked markers can be used for maker -assisted selection (MAS) and candidate gene discovery.