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ARS Home » Northeast Area » Washington, D.C. » National Arboretum » Floral and Nursery Plants Research » Research » Publications at this Location » Publication #357051
Research Project: Detection, Identification, and Characterization of New and Emerging Viral and Bacterial Diseases of Ornamental Plants

Location: Floral and Nursery Plants Research

Title: Full-length infectious clones of two new isolates of tomato mosaic virus induce distinct symptoms associated with two differential amino acid residues in 128 kDa protein

Author
item CHOI, GO-WOON - Chungnam National University
item CHO, IN-SOOK - National Institute Of Horticultural & Herbal Science (NIHHS)
item JU, HYE-KYOUNG - Chungnam National University
item HU, WEN-XING - Chungnam National University
item KIM, BORAM - Chungnam National University
item OH, JUNE-PYO - Chungnam National University
item SEO, EUN-YOUNG - Chungnam National University
item PARK, JONG-SEOK - Chungnam National University
item Domier, Leslie
item LIM, HYOUN-SUB - Chungnam National University
item Hammond, John
item SONG, KIHAK - Chungnam National University

Submitted to: Plant Pathology Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/10/2019
Publication Date: 10/8/2019
Citation: Choi, G., Cho, I., Ju, H., Hu, W., Kim, B., Oh, J., Seo, E., Park, J., Domier, L.L., Lim, H., Hammond, J., Song, K. 2019. Full-length infectious clones of two new isolates of tomato mosaic virus induce distinct symptoms associated with two differential amino acid residues in 128 kDa protein. Plant Pathology Journal. 35(5):538-542. https://doi.org/10.5423/PPJ.NT.12.2018.0286.
DOI: https://doi.org/10.5423/PPJ.NT.12.2018.0286

Interpretive Summary: Infection by plant viruses causes reductions of yield and quality in many crops, and especially horticultural crops which are pruned or otherwise handled during the growing cycle. New viruses differing in pathogenicity may arise via mutation, or by introduction of contaminated seed or planting stock, or by vectored transmission. Tomato mosaic virus (ToMV) is of increasing importance in tomato production in South Korea. Scientists in Korea collaborated with an ARS scientist in Beltsville, MD to characterize two new ToMV isolates differing in pathogenicity. They found these isolates to differ by only two amino acids. These isolates were most closely related to an isolate from Taiwan, suggesting possible importation in contaminated seed. These results suggest that seed health should be more carefully monitored to prevent possible introduction of more severe isolates of ToMV or other seedborne viruses.

Technical Abstract: In 2017 two new tomato mosaic virus (ToMV) isolates were collected from greenhouses in Buyeo Chungchungnam Do, South Korea. Full length sequences of the new ToMV isolates were cloned in T7 or joint S35and T7 promoter driven vectors, and their sequences and pathogenesis were investigated. Isolates GW1(MH507165) and GW2(MH507166) showed 99% nucleotide sequence homology, resulting in only two amino acid differences in the helicase domain, Ile(690)Thr and Val(856)Ile. The two isolates were most closely related to an isolate from Taiwan (KJ207374). Isolate GW1 (Ile690, Val856) induced a systemic hypersensitive response (sHR) in Nicotiana benthamiana and inhibited growth in Solanum lycopersicum compared to isolate GW2.