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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Hard Winter Wheat Genetics Research » Research » Publications at this Location » Publication #357359
Research Project: Genetic Improvement of Biotic and Abiotic Stress Tolerance and Nutritional Quality in Hard Winter Wheat

Location: Hard Winter Wheat Genetics Research

Title: Mapping the wheat curl mite resistance gene in the advanced wheat breeding line OK05312

Author
item ZHAO, JIXIN - Kansas State University
item MOHAMED, NADER - Kansas State University
item KHALAF, LUAAY - Kansas State University
item CHUANG, WEN-PO - Kansas State University
item ZHAO, LANFEI - Kansas State University
item SMITH, C. MICHAEL - Kansas State University
item CARVER, BRETT - Oklahoma State University
item Bai, Guihua

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/5/2019
Publication Date: 4/25/2019
Citation: Zhao, J., Mohamed, N.R., Khalaf, L., Chuang, W., Zhao, L., Smith, C., Carver, B., Bai, G. 2019. Mapping the wheat curl mite resistance gene in the advanced wheat breeding line OK05312. Theoretical and Applied Genetics. 59:1-9.

Interpretive Summary: The wheat curl mite is an important wheat pest in many wheat-growing regions worldwide. Mite feeding not only directly affects wheat yield, but also transmits Wheat streak mosaic virus. ‘OK05312’ is a new breeding line with a high level of resistance to the wheat curl mite. A high-density genetic map was constructed using progeny from the cross of the susceptible line ‘Jerry’ by ‘OK05312’. One gene for resistance was identified on chromosome 6DS corresponding to the wheat curl mite resistance gene, Cmc4. We developed two closely linked Kompetitive Allele Specific PCR (KASP) markers to the gene and those markers can be used for marker-assisted selection to develop new resistant varieties.

Technical Abstract: Wheat curl mite, Aceria tosichella Keifer, is an important wheat pest in many wheat-growing regions of Australia, Europe, the Middle East and North America. Mite feeding damage not only directly affects wheat yield, but A. tosichella also transmits wheat streak mosaic virus (WSMV). Wheat resistance to A. tosichella, therefore, helps control WSMV. ‘OK05312’ is a new advanced breeding line released from Oklahoma that shows a high level of A. tosichella resistance. To determine the quantitative trait locus (QTL) for wheat resistance to A. tosichella in OK05312, a genetic linkage map was constructed using single nucleotide polymorphism (SNP) markers derived from genotyping-by-sequencing (GBS) and a recombinant inbred line (RIL) population from the cross ‘Jerry’ × OK05312. One major QTL was identified on the short arm of chromosome 6D, which corresponds to the previously reported A. tosichella resistance gene Cmc4. This QTL explained up to 71% of the phenotypic variation and was delimited in a ~3.3 cM region by SNPs 370SNP7523 and 370SNP1639. We successfully converted 12 GBS-SNPs into kompetitive allele specific PCR (KASP) markers. Two of them tightly linked to Cmc4 were validated to be highly diagnostic in a US winter wheat natural population and can be used for marker-assisted breeding for incorporation of Cmc4 into new wheat cultivars.