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ARS Home » Pacific West Area » Kimberly, Idaho » Northwest Irrigation and Soils Research » Research » Publications at this Location » Publication #357557
Research Project: Development of Elite Sugar Beet Germplasm Enhanced for Disease Resistance and Novel Disease Management Options for Improved Yield

Location: Northwest Irrigation and Soils Research

Title: DEG-SNPs associated with resistance and susceptibility to beet curly top virus

Author
item Eujayl, Imad
item Strausbaugh, Carl

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 11/1/2017
Publication Date: 1/20/2018
Citation: Eujayl, I.A., Strausbaugh, C.A. 2018. DEG-SNPs associated with resistance and susceptibility to beet curly top virus. Plant and Animal Genome XXVI Conference, San Diego, CA. January 13,2018. W943.

Interpretive Summary:

Technical Abstract: Resistance to Beet curly top virus (BCTV-transmitted by beet leafhopper) is an essential trait for sugar beet cultivars to be grown in arid and semi-arid areas worldwide. Resistant doubled haploid line (KDH13=PI663862) and susceptible inbred line (K19-19) were utilized to identify differentially expressed genes regulating reactions to BCTV. Transcriptomic data from seven treatments of KDH13 un-infested, infested but not-infected, and infected with one of the three BCTV strains (Cal/Log, Wor, and Sev) or a combination of the three strains were analyzed against transcriptomic data of the susceptible line that was infected with the three strains. Based on 28 pair-wise comparisons, differentially expressed transcripts/genes (DEG) were identified at threshold of False-Discovery-Rate (FDR) of <0.05 and a LogFC (fold change) greater than plus/minus 2.0. BCTV resistance candidate genes included cysteine-rich receptor like proteins (CRKs), which accounted for up to 11% (171 members) of the significant DEG between resistant and susceptible lines, were aligned to discover SNPs. Twelve DEG-SNPs were identified and confirmed as associated with resistance or susceptibility between in KDH13 and K19-19. These SNPs were confirmed in a set of known resistant and susceptible breeding materials and commercial cultivars.