Complete genome sequence of a Salmonella enterica subsp. enterica serovar Fresno isolate recovered from beef cattle lymph nodes

Authors: Haley, Bradd; Smith, Timothy - Tim; Harhay, Gregory; LONERAGAN, GUY - TEXAS TECH UNIVERSITY; WEBB, HATTIE - TEXAS TECH UNIVERSITY; BUGAREL, MARIE - TEXAS TECH UNIVERSITY; KIM, SEON-WOO - UNIVERSITY OF MARYLAND; Van Kessel, Jo Ann; Harhay, Dayna

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/11/2018
Publication Date: 1/10/2019
Citation: Haley, B.J., Smith, T.P., Harhay, G.P., Loneragan, G.H., Webb, H.E., Bugarel, M., Kim, S., Van Kessel, J.S., Harhay, D.M. 2019. Complete genome sequence of a Salmonella enterica subsp. enterica serovar Fresno isolate recovered from beef cattle lymph nodes. Microbiology Resource Announcements. 8(2):e01338-18. https://doi.org/10.1128/MRA.01338-18.
DOI: https://doi.org/10.1128/MRA.01338-18

Interpretive Summary: To-date over 2000 serovars (groups) of Salmonella enterica have been described, yet little information on the ecology and virulence potential exists for most of these pathogens that can impact animals and humans. A significant amount of information exists for serovars that are frequently recovered from beef and dairy cows but little is known about the serovars that are infrequently isolated from these animals. Salmonella enterica subsp. enterica serovar Fresno is a rarely-isolated serovar but it has been occasionally isolated from livestock, humans, and produce. To gain more knowledge about Salmonella Fresno, we sequenced the genome of S. Fresno isolate 69385 which was recovered from the lymph node of a beef cattle carcass collected at slaughter. We interrogated this genome for the presence of virulence factors, antibiotic resistance genes, and its relationship to other S. enterica strains. Results of our analysis idendified the presence of multiple genomic regions, known as Salmonella pathogenicity islands (SPIs), that are involved in the infection process of humans and other animals, as well as a single antibiotic resistance gene. A phylogenetic analysis (looking at evolutionary history) indicated that this isolate is a member of a lineage that contains multiple serovars known to cause significant disease in humans, and is most closely related to other S. Fresno genomes collected inside of the United States. This genome sequence has been made publicly available in the NCBI Genbank database and researchers will use it to facilitate future work on the human and animal health risks from S. enterica carriage in cattle.

Technical Abstract: Salmonella enterica serovar Fresno is an infrequently isolated serovar whose ecology and genomic characteristics have not yet been described. To further understand the genomic characteristics of this serovar, we sequenced the complete genome of a single isolate recovered from a beef cattle lymph node at harvest.