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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Crop Bioprotection Research » Research » Publications at this Location » Publication #358353
Research Project: Development of New Production Methodologies for Biocontrol Agents and Fastidious Microbes to Improve Plant Disease Management

Location: Crop Bioprotection Research

Title: Whole genome analysis and lysine degradation genes related to osmotic stress resistance in Lysinibacillus capsisi PB300

Author
item CADENA, JOHANNA - Pathway Biologic
item SASTOQUE, LEONARDO - Pathway Biologic
item BURKETT-CADENA, MARLENY - Pathway Biologic
item Dunlap, Christopher

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/20/2019
Publication Date: 3/20/2019
Citation: Cadena, J., Sastoque, L., Burkett-Cadena, M., Dunlap, C.A. 2019. Whole genome analysis and lysine degradation genes related to osmotic stress resistance in Lysinibacillus capsisi PB300. Meeting Abstract.

Interpretive Summary:

Technical Abstract: Lysinibacillus capsisi PB300 is a growth promoting rhizobacteria of cultivated plants that has recently been described as a new species within the Lysinibacillus group. PB300 is a gram-positive, strictly aerobic, motile, rod-shaped, endospore forming bacterium originally isolated from the rhizosphere of a pepper plant in Arizona, USA. Genome sequencing and annotation revealed that PB300 has 4657 protein coding sequences (CDS) divided in 463 subsystems, 11 transfer RNA (tRNA) genes, and 4 ribosomal genes. The annotation included 1354 hypothetical proteins and 3293 proteins with functional assignments. Genomic comparison to closest species showed that PB300 possess a saccharopine dehydrogenase gene (SDH) that has been found in Lysinibacillus sphaericus and other bacterial species. This SDH gene has an essential role in the lysine catabolism pathway in higher eukaryotes and has been related to osmotic stress resistance in prokaryotes. To verify whether SDH genes are induced by osmotic stress, PB300 was grown with and without lysine at different salt concentrations, showing that presence of lysine does not have a significant effect on the bacterial concentration. However, the bacterial growth rate was extended in the lysine treatments, suggesting expression of SDH gene and the use of this pathway may be an adaptation to high salt environments. This finding is of special interest because PB300 adaptation can be exploited in an agricultural context to reassess plant growth promotion effects under stressful environmental conditions, which ultimately provides solutions for growers around the world that experience problems with plant productivity.