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ARS Home » Midwest Area » Bowling Green, Kentucky » Food Animal Environmental Systems Research » Research » Publications at this Location » Publication #358378
Research Project: Developing Safe, Efficient and Environmentally Sound Management Practices for the Use of Animal Manure

Location: Food Animal Environmental Systems Research

Title: Solid phase extraction of tylosin from cattle waste with determination by liquid chromatography mass spectrometry

Author
item CARLISLE, ANNE - Western Kentucky University
item APPALA, KEERTHI - Western Kentucky University
item CONTE, ERIC - Western Kentucky University
item KASUMBA, JOHN - Western Kentucky University
item Agga, Getahun
item Loughrin, John

Submitted to: Kentucky Academy of Sciences Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 10/1/2018
Publication Date: 11/2/2018
Citation: Carlisle, A., Appala, K., Conte, E., Kasumba, J., Agga, G.E., Loughrin, J.H. 2018. Solid phase extraction of tylosin from cattle waste with determination by liquid chromatography mass spectrometry. Kentucky Academy of Sciences Symposium. Paper No. 59.

Interpretive Summary:

Technical Abstract: Tylosin is a common antibiotic that is given to poultry, pigs, and cattle. The antibiotic not only fights against bacteria, but it also increases weight gain in livestock. Tylosin is fed to about 42% of beef calves to prevent liver abscesses. Over time however, bacteria become resistant to the antibiotic, and that resistance can pass on to humans through food consumption. The amount of antibiotic resistance an animal's bacteria has developed can be associated with the concentration of the antibiotic in the animal's waste. The purpose of this study is to establish a method to quantify tylosin in cattle waste. This was accomplished by freeze drying the waste and then performing solid phase extraction and liquid chromatography mass spectrometry. The results of this method in terms of percent recovery, detection limits, and sensitivity will be presented.