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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Bioproducts Research » Research » Publications at this Location » Publication #358548
Research Project: Domestic Production of Natural Rubber and Industrial Seed Oils

Location: Bioproducts Research

Title: Fatty acid composition and oil content of seeds from perilla germplasm of Republic of Korea

Author
item KIM, HUYN UK - Sejong University
item LEE, KYEONG-RYEOL - Jeonju University
item JEON, IN HWA - Jeonju University
item JUNG, HA EUN - Sejong University
item HEO, JAE BOK - Dong-A University
item KIM, TAE-YUN - Asia Seed
item Chen, Grace

Submitted to: Genetic Resources and Crop Evolution
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/1/2019
Publication Date: 7/6/2019
Citation: Kim, H., Lee, K., Jeon, I., Jung, H., Heo, J., Kim, T., Chen, G.Q. 2019. Fatty acid composition and oil content of seeds from perilla germplasm of Republic of Korea. Genetic Resources and Crop Evolution. 66(7):1615-1624. https://doi.org/10.1007/s10722-019-00803-8.
DOI: https://doi.org/10.1007/s10722-019-00803-8

Interpretive Summary: Perilla (Perilla frutescens (L.) var. frutescens) is an oilseed crop that produces a large amount of a-linolenic acid (ALA, 18:3), an '-3 fatty acid in seeds. Perilla is also a fragrant leafy herb with great health benefits. To develop value-added perilla crop, we surveyed the fatty acid composition and oil content of 87 germplasms accessions maintained at Republic of Korea. Our results show that ALA is the major fatty acid, ranging from 47–64%, followed by linoleic acid (LA, 18:2, an '-6 fatty acid) from 10–24% and the oleic acid (OA, 18:1, an '-9 fatty acid) from 9–20%. The seed oil content among accessions ranges from 17–42.7%. To access if the different fatty acid profile and oil content among germplasms are associated with changes in key genes, we compare the sequences encoding fatty acid desaturase 2 (FAD2) and fatty acid desaturase 3 (FAD3) responsible for LA and ALA synthesis, respectively. Besides, we examine the sequence of diacylglycerol acyltransferase 1 (DGAT1), a key enzyme in determining oil content. We do not find any changes in FAD2 or FAD3 associated with different fatty acid profiles. We discover a change in DGAT1 that might associate a low oil content in seeds.

Technical Abstract: Perilla (Perilla frutescens (L.) var. frutescens) is an oilseed crop that produces a large amount of a-linolenic acid (ALA, 18:3), an '-3 fatty acid in seeds. Perilla is also a fragrant leafy herb with great health benefits. To develop value-added perilla crop, we surveyed the fatty acid composition and oil content of 87 germplasms accessions maintained at Republic of Korea. Our results show that ALA is the major fatty acid, ranging from 47–64%, followed by linoleic acid (LA, 18:2, an '-6 fatty acid) from 10–24% and the oleic acid (OA, 18:1, an '-9 fatty acid) from 9–20%. The seed oil content among accessions ranges from 17–42.7%. To access if the different fatty acid profile and oil content among germplasms are associated with changes in key genes, we compare the sequences encoding fatty acid desaturase 2 (FAD2) and fatty acid desaturase 3 (FAD3) responsible for LA and ALA synthesis, respectively. Besides, we examine the sequence of diacylglycerol acyltransferase 1 (DGAT1), a key enzyme in determining oil content. We do not find any changes in FAD2 or FAD3 associated with different fatty acid profiles. We discover a change in DGAT1 that might associate a low oil content in seeds.