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ARS Home » Southeast Area » Dawson, Georgia » National Peanut Research Laboratory » Research » Publications at this Location » Publication #359239
Research Project: Integration of Traditional Methods and Novel Molecular Strategies for Improving Disease Resistance and Input-use Efficiency in Peanut

Location: National Peanut Research Laboratory

Title: Genome-wide transcriptome and physiological analyses provide new insights into peanut drought response mechanisms

Author
item BHOGIREDDY, SAILAJA - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India
item XAVIER, ABISHEK - New Mexico State University
item GARG, VANIKA - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India
item LEYLAND, NANCY - Food Safety Inspection Service (FSIS)
item Arias De Ares, Renee
item Payton, Paxton

Submitted to: Scientific Reports
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/7/2020
Publication Date: 3/5/2020
Citation: Bhogireddy, S., Xavier, A., Garg, V., Leyland, N., Arias De Ares, R.S., Payton, P.R. 2020. Genome-wide transcriptome and physiological analyses provide new insights into peanut drought response mechanisms. Scientific Reports. 10:4071. https://doi.org/10.1038/s41598-020-60187-z.
DOI: https://doi.org/10.1038/s41598-020-60187-z

Interpretive Summary: Drought is one of the major constraints for the peanut production worldwide and lack of legitimate understanding towards molecular mechanisms prevent the accelerated genetic improvement. In this study, composite analysis of phenotyping, physio-biochemical and transcriptome investigations decipher the underlying drought stress responsive mechanisms in peanut. Multi-season (2013 - 2015) phenotyping of 10 genotypes under water deficit irrigation (WDI) revealed C76 -16 (C-76) as best performing under WDI while Valencia (Val-C) as least. Further, RNA-seq in both genotypes deciphers the transcriptome changes under WDI. About 369 million raw reads were generated from four different libraries constructed from fully irrigated (FI) and WDI samples, out of which 329 (90.2%) filtered reads were mapped to the peanut genome. Analysis revealed the differential expression of 4508 genes, 1554 transcription factors and 514 SNPs/Indels. Further, comparative analysis revealed the expression of osmoprotectant genes like proline iminopeptidase, peroxidase, abscisic acid 8- hydroxylase and other genes like lipid transfer, and photosystem II D2 in C-76 reflecting its basal and integral tolerance mechanism. This study not only understand the response of genotypes to WDI, but also identified genes and SNPs could be deployed in development of molecular markers for drought response selection of genotypes through marker-assisted breeding.

Technical Abstract: Drought is one the main causes of economic losses in peanut. This work focuses on identifying genes, small variants in those genes called single-nucleotide polymorphisms (SNPs), and the level at which they are expresses in different peanut cultivars under drought stress. The results presented in this work are a significant progress towards breeding peanut cultivars/varieties with tolerance to drought conditions.