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ARS Home » Northeast Area » Beltsville, Maryland (BHNRC) » Beltsville Human Nutrition Research Center » Diet, Genomics and Immunology Laboratory » Research » Publications at this Location » Publication #359746
Title: Gypenosides reduced the risk of overweight and insulin resistance in C57BL/6J mice through modulating adipose thermagenesis and gut microbiota

Author
item LIU, JIE - Beijing Advanced Innovation Center For Food Nutrition And Human Health, Beijing Technology & Busine
item LI, YANFANG - Shanghai Jiaotong University
item YANG, PUYU - Shanghai Jiaotong University
item WAN, JIANCHUN - Shanghai Jiaotong University
item ZHANG, YAQIONG - Shanghai Jiaotong University
item GAO, BOYAN - Shanghai Jiaotong University
item WANG, ZIYUAN - Beijing Advanced Innovation Center For Food Nutrition And Human Health, Beijing Technology & Busine
item Wang, Thomas - Tom
item YU, LIANGLI - University Of Maryland

Submitted to: Journal of Food Science and Nutrition
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/4/2017
Publication Date: 10/25/2017
Citation: Liu, J., Li, Y., Yang, P., Wan, J., Zhang, Y., Gao, B., Wang, Z., Wang, T.T., Yu, L. 2017. Gypenosides reduced the risk of overweight and insulin resistance in C57BL/6J mice through modulating adipose thermagenesis and gut microbiota. Journal of Food Science and Nutrition. 65(42)9237-9246. https://doi.org/doi: 10.1021/acs.jafc.7b03382.
DOI: https://doi.org/10.1021/acs.jafc.7b03382

Interpretive Summary: Obesity and related medical problems are serious public health issues and are preventable by sound diet. However, the mechanisms as well as bio-active components in the diet remain unclear. This study investigated the effect of dietary gypenosides on obesity and its associated inflammation, insulin resistance and hepatic steatosis. Male obese C57BL/6J mice induced by feeding a high-fat diet (HFD) for 16 weeks were divided into a treatment group supplied with 300 mg/kg BW/d gypenosides and a control group, and fed HFD for another eight weeks. The gypenosides supplementation significantly reduced body weight gain, total plasma cholesterol and homeostasis model assessment-estimated insulin resistance (HOMA-IR) index compared with the control group. Levels of tumor necrosis factor-a, monocyte chemoattractant protein-1, and interleukin-6 were decreased in the gypenoside treatment group. Moreover, gypenosides consumption alleviated hepatic steatosis and insulin resistance possibly by promoting energy expenditure through AMPK signaling pathway and upregulating thermogenic genes in the brown and inguinal white adipocyte tissues. In addition, these metabolic changes were accompanied by an increase in the abundance of Akkermansia muciniphila in the gut microbiota. This study provide novel information on regulatory mechanisms of diet-derived bio-actives on immune responses, insulin resistance, liver steatosis, microbiome and will benefit basic and translational scientist that are studying prevention of obesity and obesity-related chronic diseases.

Technical Abstract: This study investigated the effect of dietary gypenosides on obesity and its associated inflammation, insulin resistance and hepatic steatosis. Male obese C57BL/6J mice induced by feeding a high-fat diet (HFD) for 16 weeks were divided into a treatment group supplied with 300 mg/kg BW/d gypenosides and a control group, and fed HFD for another eight weeks. The gypenosides supplementation significantly reduced body weight gain, total plasma cholesterol and homeostasis model assessment-estimated insulin resistance (HOMA-IR) index compared with the control group. Levels of tumor necrosis factor-a, monocyte chemoattractant protein-1, and interleukin-6 were decreased in the gypenoside treatment group. Moreover, gypenosides consumption alleviated hepatic steatosis and insulin resistance possibly by promoting energy expenditure through AMPK signaling pathway and upregulating thermogenic genes in the brown and inguinal white adipocyte tissues. In addition, these metabolic changes were accompanied by an increase in the abundance of Akkermansia muciniphila in the gut microbiota.