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Research Project: IPM Methods for Insect Pests of Orchard Crops

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Title: Advances in the Micro-CT procedures to study Asian citrus psyllid and Liberibacter pathogen

Author
item ALBA-TERCEDOR, JAVIER - Universidad De Granada
item Hunter, Wayne
item CICERO, JOESEPH - University Of Florida

Submitted to: Workshop Proceedings
Publication Type: Research Technical Update
Publication Acceptance Date: 11/8/2018
Publication Date: 12/11/2018
Citation: Hunter, W.B., Alba-Tercedor, J., Cicero, J.M. 2018. Advances in the Micro-CT procedures to study Asian citrus psyllid and Liberibacter pathogen. The 4th Annual Citrus Greening Solutions Meeting. December 11-12, 2018, Fort Pierce, Florida. p. 12.4.

Interpretive Summary: New methods for sample preparation improved visualizing the anatomy inside male and female adult Asian citrus psyllids. The reproductive systems of each gender has now been clearly presented. Novel images of the bacteria in the specialized organs, bacteriomes, is a major breakthrough. Use of Micro-Computed Tomography, similar to hospital CT or 'CAT' scan imaging, now makes analyses and imaging of small insects highly accurate.

Technical Abstract: New methods for sample preparation improved visualizing the anatomy inside male and female adult Asian citrus psyllids. The reproductive systems of each gender has now been clearly presented. Novel images of the bacteria in the specialized organs, bacteriomes,is a major breakthrough. Use of Micro-Computed Tomography, similar to hospital CT or 'CAT' scan imaging, now makes analyses and imaging of small insects highly accurate. New understanding of organ functions provides insights into potential biological targets for pest management.