Rare maternal and bi-parental transmission of the cucumber mitochondrial DNA reveals sorting of polymorphisms among progenies

Authors: SHEN, JIA - Zhejiang Academy Of Agricultural Sciences; SHOU, WEISONG - Zhejiang Academy Of Agricultural Sciences; ZHANG, YUEJIAN - Zhejiang Academy Of Agricultural Sciences; YUAN, GAOYA - Nanjing Agricultural University; ZHAO, YU - Nanjing Agricultural University; CHEN, JINFENG - Nanjing Agricultural University; Havey, Michael

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/1/2018
Publication Date: 1/12/2019
Citation: Shen, J., Shou, W., Zhang, Y., Yuan, G., Zhao, Y., Chen, J., Havey, M.J. 2019. Rare maternal and bi-parental transmission of the cucumber mitochondrial DNA reveals sorting of polymorphisms among progenies. Meeting Abstract. 132:1223-1233. https://doi.org/10.1007/s00122-018-03274-0.
DOI: https://doi.org/10.1007/s00122-018-03274-0

Interpretive Summary:

Technical Abstract: The mitochondrial (mt) DNA of cucumber is paternally transmitted, and it is unclear if relatively rare maternal or biparental transmission occurs. We used a mt mutant (MSC16) of cucumber to screen for maternally or biparentally transmitted mt DNA. Wild-type progenies from crosses of MSC16 as the male were selected and genotyped for indel polymorphisms in the mt DNA, and rare maternal and biparental transmission of mt polymorphisms were detected. We then used normal and droplet-digital (dd) PCR to study transmission of polymorphic mt markers across generations. Different combinations of maternal and paternal mt polymorphisms were revealed in progenies across generations, indicating that maternal regions were transmitted to progenies and can predominate. These results indicate that rare maternal or biparental transmission of mt DNA occurs in cucumber, and sorting of these polymorphisms contributes to mt DNA diversity over generations.