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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » National Germplasm Resources Laboratory » Research » Publications at this Location » Publication #361731
Research Project: Characterizing and Detecting Pathogens to Ensure Safe Exchange of Plant Germplasm

Location: National Germplasm Resources Laboratory

Title: Detection of Sugarcane striate virus and Sugarcane white streak virus in the Miami World Collection of sugarcane and related grasses

Author
item BOUKARI, WARDA - University Of Florida
item FILLOUX, DENIS - Cirad-La Recherche Agronomique Pour Le Developpe
item FENOUILLET, CATHERINE - Cirad-La Recherche Agronomique Pour Le Developpe
item DAUGROIS, JEAN-HEINRICH - Cirad-La Recherche Agronomique Pour Le Developpe
item FERNANDEZ, EMMANUEL - Cirad-La Recherche Agronomique Pour Le Developpe
item Mollov, Dimitre
item KAYE, CLAUDIA - Us Sugar Corporation
item HINCAPIE, MARTHA - University Of Florida
item Sanchez, Alex
item WANG, LIPING - University Of Florida
item WANG, JIANPING - University Of Florida
item ROUMAGNAC, PHILIPPE - Cirad-La Recherche Agronomique Pour Le Developpe
item ROTT, PHILIPPE - University Of Florida

Submitted to: Plant Pathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/28/2020
Publication Date: 5/22/2020
Citation: Boukari, W., Filloux, D., Fenouillet, C., Daugrois, J., Fernandez, E., Mollov, D.S., Kaye, C., Hincapie, M., Sanchez, A.W., Wang, L., Wang, J., Roumagnac, P., Rott, P. 2020. Detection of Sugarcane striate virus and Sugarcane white streak virus in the Miami World Collection of sugarcane and related grasses. Plant Pathology. https://doi.org/10.1111/ppa.13192.
DOI: https://doi.org/10.1111/ppa.13192

Interpretive Summary: Sugarcane is a crop affected by many pathogens including viruses. Sugarcane white streak virus (SWSV) and sugarcane striate virus (SStrV) are DNA viruses that have been reported mostly from Africa and the Indian Ocean islands. In this study, we developed a PCR detection method for these two viruses and screened the USDA-ARS sugarcane germplasm collection in Miami, FL for them. A total of 571 sugarcane plants were tested. All samples tested negative for SWSV, but SStrV was detected in 19 plants. The results confirm that SStrV is present in Florida and may necessitate screening for the virus in domestic sugarcane research and commercial breeding lines. This action would minimize the spread of SStrV in U.S. commercial sugarcane production.

Technical Abstract: Viruses in the genus Mastrevirus (family Geminiviridae), including those infecting sugarcane, have natural geographical ranges almost exclusively restricted to Africa and the Indian Ocean islands off the African coast. Only sugarcane white streak virus (SWSV) in Barbados and sugarcane striate virus (SStrV) in Florida and Guadeloupe are known to infect sugarcane in the Western hemisphere. In this study, PCR diagnostic assays were developed to detect these two viruses in sugarcane. Using these assays, occurrence of SWSV and SStrV was determined in 571 DNA samples from Saccharum species and interspecific hybrids from the Miami World Collection of sugarcane and related grasses. No plant was found infected by SWSV but SStrV was detected in 19 plants. PCR data were confirmed by sequencing amplified fragments (248 bp) that shared 93-100 % nucleotide identity with SStrV sequences from the GenBank database. Virus particles were also observed in infected plants by electron microscopy. Most plants infected by SStrV originated from Asia, thus confirming a previous hypothesis stating that this virus originated from this continent. Absence of SStrV in commercial sugarcane in Florida also suggests that this virus has not been spread in this location although infected plants have been present for more than a century.