Discovery of novel bioactive constituents from actinomycetes toward various agricultural pathogens

Authors: Kim, Seong; CHEN, YUYAN - Shenzhen Life Science And Biotechnology Association; CHEN, YUFENG - Hainan University; Pan, Zhiqiang - Peter; Wedge, David; Cantrell, Charles

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/2/2019
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Finding natural product-based solutions to help manage agriculture pests is a major global challenge due to an increased incidence of chemical resistance by agrochemical pathogens. The aim of this project was to identify new natural product-based pesticides and biopesticides for use as plant protectants or phytochemicals in plant protection and production. We focused on unique actinomycete species not previously studied as a source of antifungal constituents. Four bioactive organisms were isolated that can effectively control banana fusarium wilt disease caused by the soil-borne Fusarium oxysporum f. sp. cubense. These organisms were subsequently identified by sequence analyses of the ITS region of the rRNA genes as belonging to the species Streptomyces yunnanensis. The butanol extract of the culture filtrate from Streptomyces yunnanensis was analyzed by innovative technology and potent bioactive preparations were evaluated by various in-house rapid bioassays using an activity-directed fractionation approach to determine potential inhibitory effects towards phytopathogenic fungi included Colletotrichum fragariae, C. acutatum, and C. gloeosporioides. Further studies toward the identification of specific bioactive compounds and their detailed biochemical pathways and physiological studies are currently underway.