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ARS Home » Southeast Area » Raleigh, North Carolina » Plant Science Research » Research » Publications at this Location » Publication #363867
Research Project: Genetics of Disease Resistance and Food Quality Traits in Corn

Location: Plant Science Research

Title: Quantifying MAMP-induced production of reactive oxygen species in sorghum and maize

Author
item SAMIRA, ROZALYNNE - North Carolina State University
item ZHANG, XINYE - North Carolina State University
item KIMBALL, JENNIFER - University Of Missouri
item CUI, YAYA - University Of Missouri
item STACEY, GARY - University Of Missouri
item Balint-Kurti, Peter

Submitted to: Bio-protocol
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/1/2019
Publication Date: 7/20/2019
Citation: Samira, R., Zhang, X., Kimball, J., Cui, Y., Stacey, G., Balint Kurti, P.J. 2019. Quantifying MAMP-induced production of reactive oxygen species in sorghum and maize. Bio-protocol. https://doi.org/10.21769/BioProtoc.3304.
DOI: https://doi.org/10.21769/BioProtoc.3304

Interpretive Summary: We describe methods to measure the basal defense response in maize and sorghum.

Technical Abstract: Plants have the ability to recognize microbe associated molecular profiles (MAMPS) and mount a defense response. The level of the MAMP response can vary depending on genetic and environmental factors. The most commonly studied MAMPs are flg22, an epitope from bacterial flagellin, and chitin, a component of the fungal cell wall. Protocols for measuring ROS production elicited by flg22 and chitin in maize and sorghum are described.