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ARS Home » Southeast Area » Fort Pierce, Florida » U.S. Horticultural Research Laboratory » Subtropical Plant Pathology Research » Research » Publications at this Location » Publication #364076
Research Project: Mitigating High Consequence Domestic, Exotic, and Emerging Diseases of Fruits, Vegetables, and Ornamentals

Location: Subtropical Plant Pathology Research

Title: Sequence analysis of the medium and small RNAs of impatiens necrotic spot virus reveals segment reassortment but not recombination

Author
item ADEGBOLA, RAPHAEL - Washington State University
item MARSHALL, SPENCER - Washington State University
item BATUMAN, OZGUR - University Of Florida
item ULLMAN, DIANE - University Of California, Davis
item GILBERTSON, ROBERT - University Of California, Davis
item Adkins, Scott
item NAIDU, RAYAPATI - Washington State University

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/31/2019
Publication Date: 9/5/2019
Citation: Adegbola, R.O., Marshall, S.H., Batuman, O., Ullman, D.E., Gilbertson, R.L., Adkins, S.T., Naidu, R.A. 2019. Sequence analysis of the medium and small RNAs of impatiens necrotic spot virus reveals segment reassortment but not recombination. Archives of Virology. https://doi.org/10.1007/s00705-019-04389-5.
DOI: https://doi.org/10.1007/s00705-019-04389-5

Interpretive Summary: Impatiens necrotic spot virus (INSV) is an economically important pathogen of ornamental and vegetable crops. Complete genome segment nucleotide sequences were determined and analyzed. High levels of nucleotide identities were observed with corresponding sequences available in public databases. Phylogenetic analysis revealed the first evidence of intra-species segment reassortment in INSV. These results will lead to a better understanding of INSV epidemiology and be useful for Extension, regulatory and research personnel.

Technical Abstract: Impatiens necrotic spot virus (INSV) is an economically important pathogen of ornamental and vegetable crops. The complete nucleotide sequences of the medium (M)- and small (S)-RNA genome segments were determined for twelve INSV isolates collected from eight plant species. The M- and S-RNAs of these twelve isolates shared nucleotide identities of 97–99% and 93–98%, respectively, with corresponding full-length sequences available in public databases. Phylogenetic analysiess based on the M- or S-RNA sequences showed differences in the phylogenetic position of some isolates suggesting intra-species segment reassortment. In contrast, phylogenetic relationships of INSV isolates based on individual and concatenated sequences of genes encoded by M- or S-RNAs revealed no discordance indicating that phylogenetic incongruence is not the result of intra-segment recombination events. Although described in other members of the genus Orthotospovirus, this is the first report of intra-species segment reassortment in INSV. The results of this study will lead to a better understanding of the epidemiology of INSV for improved crop management.