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ARS Home » Pacific West Area » Hilo, Hawaii » Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center » Tropical Plant Genetic Resources and Disease Research » Research » Publications at this Location » Publication #364893
Research Project: Genetic Improvement and Sustainable Production Systems for Sub-tropical and Tropical Crops in the Pacific Basin

Location: Tropical Plant Genetic Resources and Disease Research

Title: Transcriptome analysis of a Radopholus similis population isolated in Hawai'i

Author
item VIEIRA, PAULO - Virginia Tech
item Myers, Roxana
item Mello, Cathy
item Matsumoto Brower, Tracie
item Kamo, Kathryn

Submitted to: Journal of Nematology
Publication Type: Abstract Only
Publication Acceptance Date: 6/28/2019
Publication Date: 12/31/2019
Citation: Vieira, P., Myers, R.Y., Mello, C.L., Matsumoto Brower, T.K., Kamo, K.K. 2019. Transcriptome analysis of a Radopholus similis population isolated in Hawai'i. Journal of Nematology. 51:43. https://doi.org/10.21307/jofnem-2019-065.
DOI: https://doi.org/10.21307/jofnem-2019-065

Interpretive Summary:

Technical Abstract: The burrowing nematode, Radopholus similis, is an economically important pathogen that inflicts damage and yield loss to a wide range of crops. This migratory endoparasite is widely distributed in Hawaii and causes extensive destruction to the root systems of banana and anthurium. In banana, burrowing nematode infestations can lead to decline and toppling of large plants. Infected anthuriums become severely stunted resulting in smaller flowers and yield reductions of up to 50%. To obtain insight into the transcriptome of this species, we used Illumina mRNA sequencing analysis of a mixed population originally collected from a commercial anthurium farm in Hilo, Hawaii. Over 106 million paired end reads were obtained, and a de novo transcriptome assembly resulted in a total of 44,478 transcripts. Homology searches showed significant hit matches to 57.6% of the total number of transcripts using different public databases. Using different comparison analyses, several candidate effector genes were identified, both pioneer genes and transcripts with similarities to other nematode parasitism genes. A small set of candidate effector genes were localized within the esophageal glands of the nematode by in situ hybridization. These analyses provide additional transcriptome data for a migratory and economically important plant-parasitic nematode.